| 基于LC/MS的血清胆汁酸代谢轮廓快速定量方法及其在宫颈癌研究中的应用 |
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| 引用本文： | 陈静,单圆鸿,路鑫,万小平,许国旺.基于LC/MS的血清胆汁酸代谢轮廓快速定量方法及其在宫颈癌研究中的应用[J].质谱学报,2009,30(Z1):109-110. |
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| 作者姓名： | 陈静 单圆鸿 路鑫 万小平 许国旺 |
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| 作者单位： | 1. 中国科学院大连化学物理研究所，分离分析重点实验室，辽宁 大连 116023； 2. 上海交通大学附属第一人民医院妇产科，上海 200080 |
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| 摘 要： |
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Rapid Quantification of Serum Bile Acid Profiles Based on High Performance Liquid Phase Chromatography-Mass Spectrometry and Its Application in Woman Cervical Cancer |
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| CHENJing,SHANYuan-hong,LUXin,WANXiao-ping,XUGuo-wang.Rapid Quantification of Serum Bile Acid Profiles Based on High Performance Liquid Phase Chromatography-Mass Spectrometry and Its Application in Woman Cervical Cancer[J].Journal of Chinese Mass Spectrometry Society,2009,30(Z1):109-110. |
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| Authors: | CHENJing SHANYuan-hong LUXin WANXiao-ping XUGuo-wang |
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| Affiliation: | 1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. Department of Obstetrics and Gynaecology, the First People’s Hospital, Shanghai Jiaotong University, Shanghai 200080, China |
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| Abstract: | A simple and rapid method for the analysis of 12 bile acids was developed based on LC/MS. Quantification was finished in 7 min. For different bile acids, the detection limits ranged from 0.006 1 to 0.052 4 mg L－1. The linear range was 2-3 magnitudes. The precisions of pretreatment for each bile acid were CVs＜10% except GCA. The developed method was applied to the analysis of human serums consisting of 22 cervical cancer women and 25 healthy women. The results revealed that GUDCA, GCDCA, TUDCA, TCDCA, TDCA, UDCA, CDCA and GCA were significantly increased in cervical cancer women. |
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| Keywords: | LC/MS metabolitesprofiling bileacids quantification |
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