| pEgr-MDA7重组质粒辐射诱导特性的检测 |
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| 引用本文： | 吴丛梅,黄天华,吴德生,谢庆东,徐小虎.pEgr-MDA7重组质粒辐射诱导特性的检测[J].癌变．畸变．突变,2006,18(5):381-383. |
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| 作者姓名： | 吴丛梅 黄天华 吴德生 谢庆东 徐小虎 |
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| 作者单位： | 1．汕头大学医学院 生殖医学研究中心，广东 汕头 515041；2．汕头大学法医学教研室，广东 汕头 515041 |
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| 基金项目： | 国家自然科学基金资助项目(No.30210103904)，广东省科技计划项目资助(No.2003C30304) |
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| 摘 要： | 背景与目的：克隆人MDA7 cDNA基因，构建pEgr-MDA7重组质粒并检测其在人食管癌细胞系EC9706中经辐射诱导后mRNA水平。材料与方法：从人外周血mRNA中克隆人MDA7 cDNA基因，连接到Egr-1启动子的下游构建成pEgr- MDA7重组质粒，利用脂质体介导转染人EC9706细胞，用定量PCR方法检测不同剂量X射线照射后被转染细胞中MDA7的mRNA水平。结果：测序结果证实MDA7 cDNA基因序列正确，酶切鉴定证实pEgr-MDA7重组质粒构建正确。被pEgr- MDA7重组质粒转染的人食管癌EC9706细胞经不同剂量X射线照射后，MDA7基因mRNA水平均高于未照射组。结论：X射线可诱导pEgr- MDA7重组质粒在人EC9706细胞中表达增强。
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| 关 键 词： | MDA-7基因 pEgr-MDA7重组质粒 辐射 |
| 文章编号： | 1004-616X（2005）05-0381-03 |
| 收稿时间： | 2005-01-28 |
| 修稿时间： | 2005-04-20 |
The Irradiation-Induced Property of pEgr-MDA7 Recombinant Plasmid |
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| WU Cong-mei,HUANG Tian-hua,Wu De-sheng,XIE Qing-dong,XU Xiao-hu.The Irradiation-Induced Property of pEgr-MDA7 Recombinant Plasmid[J].Carcinogenesis,Teratogenesis and Mutagenesis,2006,18(5):381-383. |
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| Authors: | WU Cong-mei HUANG Tian-hua Wu De-sheng XIE Qing-dong XU Xiao-hu |
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| Affiliation: | 1.Research Center of Reproductive Medicine,Shantou University Medical College, Shantou 515041, Guangdong,China;2.Department of Forensic Medicine,Shantou University Medical College,Shantou 515041,Guang Dong,China |
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| Abstract: | BACKGROUND & AIM: To clone human MDA7 cDNA gene,construct pEgr-MDA7 plasmid,and measure its mRNA level in esophageal squamous EC9706 cells induced by irradiation. MATERIAL AND METHODS: Human MDA7 cDNA gene was isolated from peripheral blood and was then ligated to downstream of Egr-1 promoter to construct pEgr-MDA7 plasmid. The recombinant plasmids were transfected into EC9706 cells with liposome,then MDA7 mRNA level of after different doses of X-ray irradiation was quantified by PCR. RESULTS: Sequencing and restriction enzyme digestion were performed ensuring that MDA7 cDNA gene cloning and construction of pEgr-MDA7 were correct. MDA7 mRNA level in cells transfected with pEgr-MDA7 induced by different doses of irradiation was higher than that of sham-irradiation group. CONCLUSION: X-ray could induce and enhance expression of pEgr-MDA7 gene in EC9706 cells. |
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| Keywords: | melanoma differentiation associated gene pEgr-MDA7 recombinant plasmid irradiation |
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