| 四季蜜龙眼花芽形成相关基因SAM的克隆 |
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| 引用本文： | 王晓航,陈清西,郑梦娇.四季蜜龙眼花芽形成相关基因SAM的克隆[J].现代农业科技,2010(23). |
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| 作者姓名： | 王晓航 陈清西 郑梦娇 |
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| 作者单位： | 福建农林大学园艺学院; |
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| 摘 要： | 以四季蜜龙眼花芽为材料,进行了总RNA的提取与纯化,应用RT-PCR技术扩增其3′端序列,经测序和拼接,获得SAM3′末端,共1 253 bp;序列分析表明,与杨树SAM基因同源性达到96%,为龙眼成花机理的深入研究奠定了基础。
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| 关 键 词： | 四季蜜龙眼 成花相关基因 SAM基因 |
Cloning of SAM Gene Relate to Floral Bud Formation in Dimocarpus longan Lonr.cv Sijimi |
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| Authors: | WANG Xiao-hang CHEN Qing-xi ZHENG Meng-jiao |
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| Affiliation: | WANG Xiao-hang CHEN Qing-xi ZHENG Meng-jiao(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002) |
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| Abstract: | The extraction and purification of the RNA of flower bud of Dimocarpus longan Lour.cv Sijimi were conducted.Utilizing RT-PCR technique to expand 3' end sequence of SAM,testing its sequence and putting them together,the 3'end of SAM at the amount of 1 253 bp was attained.The nucleotide sequence comparison with the SAM gene of Populus trichocarpa showed that identity was higher than 96%,which would provide the basis to further study on the mechanism of blossom formation. |
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| Keywords: | Dimocarpus longan Lour cv Sijimi the relevant gene to floral bud formation SAM gene |
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