| HPLC法测定胃痛宁片中咖啡酸的含量 |
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| 引用本文： | 沈晓萌,黄艳萍.HPLC法测定胃痛宁片中咖啡酸的含量[J].海峡药学,2017,29(2). |
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| 作者姓名： | 沈晓萌 黄艳萍 |
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| 作者单位： | 广东食品药品职业学院 广州 510520 |
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| 基金项目： | 广东省科技计划项目2011B020301007 |
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| 摘 要： | 目的 建立胃痛宁片中咖啡酸的含量方法,以便有效控制胃痛宁片的质量.方法 采用HPLC法,色谱柱为Diamonsil C18(250mm×4.6mm,5μm);流动相:甲醇-0.2%乙酸水溶液(23∶77);流速为1.0mL·min-1;柱温:40℃;检测波长为322nm.结果 咖啡酸在3.28~43.74μg·mL-1范围内呈良好线性关系(r=0.9999).平均加样回收率为99.82%,RSD为0.65%(n=6).结论 建立的方法准确,简便,重现性好,可用于胃痛宁片的质量控制.
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| 关 键 词： | 胃痛宁片 咖啡酸 高效液相色谱法 |
Determination of Caffeic Acid in Weitongning Tablets by HPLC |
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| SHEN Xiao-meng,HUANG Yan-ping.Determination of Caffeic Acid in Weitongning Tablets by HPLC[J].Strait Pharmaceutical Journal,2017,29(2). |
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| Authors: | SHEN Xiao-meng HUANG Yan-ping |
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| Abstract: | OBJECTIVE To establish the method for determination of Caffeic Acid in Weitongning Tablets by HPLC.METHODS HPLC was developed to determine Caffeic Acid with UV absorptions at 322nm.Its Mobile phase was co nsisted of-2% of methanol glacial acetic acid (23∶77).The flow rat e was 1.0m L·min-1.The column temperature was 40℃.RESULTS Caffeic Acid showed a good linear relationship in the range of 3.28~43.74μg·mL-1 (r=0.9999),the average recovery was 99.82%,RSD was 0.65% (n=6).WTH ZCONCLUSION The established method is simple,accurate and reproducib le.It can be used for the quality control of Weitongning Tablets. |
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| Keywords: | Weitongning Tablets Caffeic acid HPLC |
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