反义凝血酶受体ODNs对大鼠血管平滑肌细胞增殖的影响

目的 :为研究凝血酶受体在血管平滑肌细胞增殖中的作用 ,探讨反义凝血酶受体ODNs对大鼠血管平滑肌细胞增殖的影响 .方法 :通过培养SD大鼠胸主动脉血管平滑肌细胞 ,以3 H -TdR掺入率作为评价反义凝血酶受体ODNs抑制血管平滑肌细胞增殖的指标 ,用反转录聚合酶链反应检测反义凝血酶受体ODNs 抑制血管平滑肌细胞凝血酶受体mRNA的表达 ,用Western -Blot检测反义凝血酶受体ODNs抑制血管平滑肌细胞凝血酶受体蛋白质的表达 .结果 :反义凝血酶受体ODNs明显抑制血管平滑肌细胞的增殖 (与对照组相比 ,P <0 0 5 ) ,明显抑制血管平滑肌细胞的凝血酶受体mRNA和蛋白的表达 .结论 :反义凝血酶受体ODNs明显抑制血管平滑肌细胞的增殖 ,其抑制血管平滑肌细胞的增殖是通过抑制凝血酶受体基因的表达 ,特别是通过抑制DNA、mRNA和蛋白的表达来完成 .

Effects of Antisense ODNs of Thrombin Receptor on the Proliferation of Rat Vascular Smooth Muscle Cell

Objective: to study the effects of thrombin receptor on the proliferation of rat vascular smooth muscle cells, probe the mechanism of antisense ODNs of thrombin receptor restrained the proliferation of rat vascular smooth cells. Methods: Taking the incoporation of 3H-thymidine as the target to evaluate vascular smooth muscle cells proliferation; Antisense ODNs of thrombin receptor restrained the expression of mRNA of thrombin receptor in vascular smooth cells was detected with RT-PCR; Antisense ODNs of thrombin receptor restrained the expression of protein of thrombin receptor was detected by Western-blot. Results: Antisense ODNs of thrombin receptor restrained vascular smooth muscle cells proliferation markedly (compared with control P<0.05), as well as expression of mRNA and protein of thrombin receptor. Conclusion: Antisense ODNs of thrombin receptor can restrain SD rat vascular smooth muscle cells proliferation markedly, which is resulted from restraining gene expression of thrombin receptor.