一品红愈伤组织的诱导与分化

以一品红的茎段、叶片、苞片及叶柄作外植体接种于Ms附加不同激素的培养基中,研究愈伤组织的诱导效果以及不同激素组合对愈伤组织分化出芽的影响。结果表明,在起始培养中以Ms BA 0．1 mg／L NAA0．1mg／L 2,4-D 2 mg／L的效果最好,最易诱导出愈伤组织,其中苞片的诱导效果优于其它外植体。在BA与NAA不同浓度组合中,茎段的诱导效果最好,其次是叶柄和苞片,叶片的诱导效果较差。愈伤组织分化产生芽的频率较低,继代培养中易出现各种颜色的次生代谢物。

Induction and Differentiation of Callus in Euphorbia Pulcherrima

The stem, leaf, bracteal leaf and petiole of the Euphorbia pulcherrima were used as explants to inoculate at the culture medium which were the combinations of MS added different concentration of BA, NAA and 2,4 -D. The results suggested that the medium of MS BA0. 1mg/L NAA0. 1mg/L 2,4 - D2mg/L had the best induction effects, especially in the bracteal leaf during the primary culture. In MS medium supplemented different concentration BA and NAA, the best induction effect of callus was the stem, the next was the petiole and the bracteal leaf, and the lowest was the leaf. The differentiation rate of adventitious buds from callus was low in all experimental media. During subculture of callus, secondary metabolism products with different colors would produce from the callus easily.