原花青素对肺癌SPC-A-1细胞X射线放射增敏作用

目的探讨葡萄籽原花青素提取物对肺腺癌SPC-A-1细胞X射线放疗的增敏作用以及其作为放疗增敏剂的可能机制。方法应用MTT法和集落形成法研究原花青素的放疗增敏效应,单因素方差分析确定增敏剂量和增敏作用,单击多靶曲线拟合计算增敏比及相关参数。流式细胞仪检测药物增敏后细胞凋亡和细胞周期的变化。结果 MTT检测结果表明,单独应用原花青素对SPC-A-1的抑制作用较弱,IC50大于400μg/ml;细胞药物处理后对X射线的敏感性增加,单因素方差分析表明药物和X射线有协同作用（P〈0.05）,100μg/ml时表现出增敏效应;集落形成结果表明,100μg/ml药物对X射线增敏作用明显,增敏比为2.56。流式细胞术检测发现试验组细胞出现了明显的凋亡峰,提示亚二倍体DNA含量的细胞数量明显增加,细胞凋亡率（28.2%）。实验显示原花青素可以诱导细胞凋亡,并将细胞阻滞于G1期。结论葡萄籽原花青素对肺腺癌SPC-A-1细胞有显著的放疗增敏作用,其机制可能是通过抑制细胞的增殖,G1期阻滞,诱导细胞凋亡。

The Radiosensitizing Effects of Grape Seed Procyanidin Extract on Lung Cancer Cell Line SPC-A-1

Objective To study the radiosensitizing effects of grape seed procyanidin extract on lung cancer cell line SPC-A-1 in vitro and try to explore its mechanism.Methods The radiosensitizing effect was detected by clone formation experiment and MTT methods.The radiosensitizing dose of anthocyanidin was determined by single-factor analysis of variance.The ratio of sensitization and relevant parameters were calculated by the multi-target/single-hit model.Flow cytometry（FCM）was used to measure cell cycle and apoptosis in radiotherapy alone group and radiosensitizer group.Results The results of MTT indicated that the inhibition effect on SPC-A-1 was slight when only using procyanidin,and the IC50 greater than 400 μg /ml.Though,after the lung cancer cell line was administrated by procyanidin,the sensitivity of X-ray radiotherapy was increased.The study showed that the procyanidin and X-ray has synergistic effects（P0.05）,the dose of 100 μg /ml has the significant sensitizing effect.Flow cytometry analysis showed that procyanidin induced apoptosis of SPC-A-1 cells（The apoptotic rate were 28.2%）.Conclusion Grape seed procyanidin extract has the significant sensitizing effect on radiotherapy in lung adenocarcinoma cells SPC-A-1.GSEP inhibits the proliferation of malignant human Lung adenocarcinoma cancer cells and induces their apoptosis.