| 苏丹黑B染色处理细胞技术在快速检测微藻油脂含量中的应用 |
| |
| 引用本文： | 林汝榕,邢炳鹏,蔡文旋,柯秀蓉,林锡煌.苏丹黑B染色处理细胞技术在快速检测微藻油脂含量中的应用[J].台湾海峡,2011,30(2):292-298. |
| |
| 作者姓名： | 林汝榕 邢炳鹏 蔡文旋 柯秀蓉 林锡煌 |
| |
| 作者单位： | 国家海洋局海洋大气化学与全球变化重点实验室、国家海洋局第三海洋研究所,福建,厦门,361005 |
| |
| 基金项目： | 国家海洋公益性行业科研专项资金资助项目 |
| |
| 摘 要： | 探讨了采用苏丹黑B染料染色处理微藻细胞技术估测微藻油脂含量的可行性.结果表明,微藻细胞经苏丹黑B染色处理后在645 nm光波长处有最大吸收峰值A645,微藻油脂含量与经苏丹黑B染色处理后微藻细胞的吸光值A645呈明显的相关性.建立了考察的7种微藻油脂含量与吸光值A645的线性回归方程,线性关系显著（p〈0.01）.基于藻湿重,微藻油脂含量的线性范围为1.79~39.63 mg/g,估算的相对偏差〈10%.因而,采用苏丹黑B染色处理微藻细胞技术适宜用来检测微藻的油脂含量.
|
| 关 键 词： | 海洋生物学 微藻油脂含量 苏丹黑B 吸光值 快速检测 回归分析 |
Rapid detection of microalgal lipid content through Sudan black B staining |
| |
| LIN Ru-rong,XING Bing-peng,CAI Wen-xuan,KE Xiu-rong,LIN Xi-huang.Rapid detection of microalgal lipid content through Sudan black B staining[J].Journal of Oceanography In Taiwan Strait,2011,30(2):292-298. |
| |
| Authors: | LIN Ru-rong XING Bing-peng CAI Wen-xuan KE Xiu-rong LIN Xi-huang |
| |
| Affiliation: | LIN Ru-rong,XING Bing-peng,CAI Wen-xuan,KE Xiu-rong,LIN Xi-huang(Key Laboratory of Global Change and Marine-Atmospheric Chemistry,Third Institute of Oceanography,SOA,Xiamen 361005,China) |
| |
| Abstract: | With continually rising fuel prices,biofuel as an alternative fuel is attracting more and more attention.Because of the high lipid content and the rapid increase in biomass,microalgae that grow in aquatic environments and use light and carbon dioxide to create biomass have been recognized as potentially good material sources for biofuel production in the future.Utilization of the microalgal lipid to produce biofuel has a promising future.Rapid and effective detection of the microalgal lipid content is impor... |
| |
| Keywords: | marine organisms lipid content of microalgae Sudan black B absorbance rapid detection regression analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
