慢性肾炎患者血清IL-2、IL-6及其受体水平变化研究

目的　研究慢性肾炎 (Chronicglomerulonephritis,CGN)患者血清IL - 2、IL - 6及其受体水平变化与意义。方法　应用细胞生物学方法测定 77例CGN患者外周血IL - 2、IL - 6及应用ELISA方法测定sIL - 2R、sIL -6R水平 ,以及应用免疫荧光法分析患者外周血淋巴细胞mL - 2R表达。结果　 77例CGN患者外周血sIL - 2R、IL - 6、sIL - 6R含量均显著升高 (P <0 .0 0 0 1) ,而IL - 2明显降低 (P <0 .0 0 0 1) ;mIL - 2R无明显变化。直线相关分析显示IL - 6与BUN呈正相关 (P <0 .0 0 5 ) ,sIL - 6R与Cr呈正相关 (P <0 .0 0 5 ) ;sIL - 2R与BUN、Cr呈正相关 (P <0 .0 5及P <0 .0 0 5 ) ,与尿蛋白定量呈负相关 (P <0 .0 5 )。结论IL - 2、sIL - 2R、IL - 6R可能与了CGN的发病 ,并与该病严重程度及预后有关 ;CGN患者IL - 2水平降低可能与患者细胞免疫功能低下相关

Alteration on the Serum IL-2、IL-6 and Receptors Levels in Patients with Chronic Glomerulonephritis

Objective To investigate the alteration and clinical significance of serum IL-2?IL-6 and receptors levels in Patients with Chronic Glomerulonephritis (CGN). Methods Serum IL-2? IL-6 levels were determined by the bioassay methods, Serum sIL-2R? sIL-6R were determined by ELISA analysis, mIL-2R were deter-mined by immunofluorescence assay in patient with CGN,and the data were compared with that in the normal controls group.Results It was found that the levels of serum IL-6? sIL-2R and sIL-6R were significantly higher in CGN than that in controls( P < 0.0001 ), respectively. While serum IL-2 markedly lower in CGN than in the controls( P <0.0001)and mIL-2R in CGN have no apparent difference with that in the controls. There was a postive correlation between IL-6 and BUN( P <0.005), positive correlation between sIL6R and SCR.( P <0.005),While there were positive correlation between sIL-2R and BUN. SCr( P<0.05;P <0.005) respectively, negative corre-lation between sIL-2R and urinary protein ( P <0.05). Conclusion IL-2?sIL-2R?IL-6?sIL-6R play an important role in the pathogenesis of CGN, could reflect severity degree and prognosis of CGN. While low levels of IL-2 attributes to cell immune states of patients with CGN was depressed.