| 雌激素对谷氨酸诱导大鼠脑皮质细胞凋亡的影响 |
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| 引用本文： | 杨卫民,周农,施雪英.雌激素对谷氨酸诱导大鼠脑皮质细胞凋亡的影响[J].安徽卫生职业技术学院学报,2010,9(5):70-71. |
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| 作者姓名： | 杨卫民 周农 施雪英 |
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| 作者单位： | 安徽医科大学第一附属医院,合肥,230022 |
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| 基金项目： | 安徽省教育厅自然科学基金资助项目 |
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| 摘 要： | 目的：观察雌激素对谷氨酸诱导离体皮层神经元凋亡的作用,探讨雌激素的神经保护作用机制。方法：原代培养大鼠大脑皮质细胞,分为正常对照组、谷氨酸组和谷氨酸＋雌激素组,采用TUNEL和免疫细胞化学检测等方法,分析雌激素对谷氨酸诱导神经元凋亡的作用。结果：谷氨酸可明显诱导神经元凋亡,凋亡指数为34.6%;雌激素能明显拮抗谷氨酸的凋亡毒性作用,凋亡指数下降为18.4%（与谷氨酸组相比P〈0.05）;免疫组化结果显示,雌激素组bcl-2表达阳性细胞明显增加,bax蛋白表达受到抑制。结论：雌激素具有神经保护作用,作用机制可能通过影响凋亡相关基因的表达来实现。
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| 关 键 词： | 雌激素 细胞凋亡 bcl-2 bax |
Effect of estrogens on Glutamate-induced apoptosis and expression of apoptosis gene of bcl-2,bax in rat cortical neurons |
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| YANG Wei-min,ZHOU Nong,SHI Xue-ying.Effect of estrogens on Glutamate-induced apoptosis and expression of apoptosis gene of bcl-2,bax in rat cortical neurons[J].Journal of Anhui Heaith Vocational & Technical College,2010,9(5):70-71. |
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| Authors: | YANG Wei-min ZHOU Nong SHI Xue-ying |
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| Affiliation: | Department of Neurology,Fist Affiliated Hospital of Anhui Medical University,Hefei 230022,Anhui YANG Wei-min,ZHOU Nong,SHI Xue-ying |
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| Abstract: | Objective:To observe the neuroprotection role and its mechanism of estrogens on apoptosis induced by Glutamate in primary rat cortical neuronal culture.Methods:Using primary rat cortical neuronal culture,TUNEL and immunocytochemistry were carried to assess the effect of estrogens on Glutamate-induced neuron apoptosis and the expression of bcl-2,bax.Results:Estrogens protects against Glutamate-induced neuronal apoptosis,higher expression of bcl-2 and lower expression of bax were also found.Conclusions:Estrog... |
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| Keywords: | bcl-2 bax |
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