| 表达HIV-1 gag-gp120嵌合基因的DNA疫苗在小鼠体内的免疫应答 |
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| 引用本文： | 江文正,金宁一,李子键,张立树.表达HIV-1 gag-gp120嵌合基因的DNA疫苗在小鼠体内的免疫应答[J].中国免疫学杂志,2004,20(7):451-453. |
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| 作者姓名： | 江文正 金宁一 李子键 张立树 |
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| 作者单位： | 1. 解放军军需大学全军基因工程重点实验室,长春,130062
2. 吉林大学第一医院,长春,130023 |
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| 基金项目： | 国家“8 63”基金资助 (基金批准号 2 0 0 1AA2 15 0 3 1) |
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| 摘 要： | 目的 :检测表达HIV 1gag gp12 0嵌合基因的DNA疫苗在小鼠体内的免疫应答效果。方法 :将真核表达质粒pVAXGE肌肉注射BALB C小鼠 ,观察免疫小鼠脾T淋巴细胞亚群的数量、特异性CTL杀伤率及小鼠免疫后不同时间点血清中IgG抗体滴度。结果 :重组质粒pVAXGE免疫组小鼠脾淋巴细胞进行了增殖 ,脾特异性CTL杀伤率显著高于对照组 (P <0 0 1) ;小鼠免疫后于第 8周血清抗体达到最高。结论 :表达HIV 1gag gp12 0嵌合基因的DNA疫苗质粒可诱导BALB C小鼠发生免疫应答
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| 关 键 词： | HIV-1 gag-gp120 DNA疫苗 免疫应答 |
| 文章编号： | 1000-484X(2004)07-0451-03 |
Immune response in mice innoculated by NDA vaccine expressing chimeric gene gag-gp120 of HIV-1 |
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| JIANG Wen Zheng,JIN Ning Yi,LI Zi Jian,ZHANG Li Shu.Key Laboratory of Genetic Engineering of PLA,The Quartermaster University of PLA,Changchun ,China.Immune response in mice innoculated by NDA vaccine expressing chimeric gene gag-gp120 of HIV-1[J].Chinese Journal of Immunology,2004,20(7):451-453. |
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| Authors: | JIANG Wen Zheng JIN Ning Yi LI Zi Jian ZHANG Li ShuKey Laboratory of Genetic Engineering of PLA The Quartermaster University of PLA Changchun China |
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| Affiliation: | JIANG Wen Zheng,JIN Ning Yi,LI Zi Jian,ZHANG Li Shu.Key Laboratory of Genetic Engineering of PLA,The Quartermaster University of PLA,Changchun 130062,China |
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| Abstract: | Objective:To detect the immune response in mice inoculated by DNA vaccine expressing chimeric gene gag gp120 of HIV 1.Methods:After BALB/C mice were immunized by the eukaryotic expression plasmid pVAXGE,the numbers of T lymphocyte subgroups of spleen,the killing rate of specific CTL from the immunized mice and the titers of serum antibodies during the different time were observed.Results:The lymphocytes of the immunized group by recombinant plasmid pVAXGE proliferated,the specific CTL killing rate of spleen was higher significantly than those of the control group( P <0 01).The peak titers of the serum antibodies reached at the eighth week after the mice were immunized.Conclusion:The immune response in BALB/C mice can be elicited by DNA vaccine plasmid expressing chimeric gene gag-gp120 of HIV-1. |
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| Keywords: | HIV-1 gag-gp120 DNA vaccine Immune response |
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