| 反相高效液相色谱法测定强筋健骨颗粒中淫羊藿苷的含量 |
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| 引用本文： | 梁文能,肖毓芳,丘振文.反相高效液相色谱法测定强筋健骨颗粒中淫羊藿苷的含量[J].国际医药卫生导报,2009,15(8):70-72. |
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| 作者姓名： | 梁文能 肖毓芳 丘振文 |
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| 作者单位： | 广州中医药大学第一附属医院,510405 |
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| 摘 要： | 目的建立复方制剂强筋健骨颗粒中的有效成分淫羊藿苷的含量测定方法。方法采用高效液相色谱法，色谱条件：色谱柱Kromasil Cus（4．6mm×250mm，5μml以乙腈-水（25：75）为流动相；检测波长：270nm；流速：1．Oml／min；柱温：25℃。结果本法可用来测定强筋健骨颗粒中淫羊藿苷的含量。淫羊藿苷在0．01949—0．3898μg范围内成良好线性关系，y=0．9998，平均回收率为99．90％，RSD为1．11％。结论本测定方法简便可行、重复性好，可用于本制剂中有效成分淫羊藿苷的含量测定。
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| 关 键 词： | 强筋健骨颗粒 淫羊藿苷 HPLC |
Detecting of Icariin in qiangjinJiangu granulas by RP-HPLC |
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| LIANG Wenneng,XIAO Yufang,QIU Zhenwen.Detecting of Icariin in qiangjinJiangu granulas by RP-HPLC[J].International Medicine & Health Guidance News,2009,15(8):70-72. |
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| Authors: | LIANG Wenneng XIAO Yufang QIU Zhenwen |
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| Affiliation: | .(The First Affilated Itospital of Guangzhou University of TCM, Cuangzhou 510405, China) |
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| Abstract: | Objective:To detect the contents of lcariin in QiangjinJiangu granulas.Methods The HPLC method was established for the determination of Icariin in Qiangjindiangu granulas.The columu is Kromasi I C18 (4.6mm × 250mm,5μm),mobile phase consisting of acetonitrile-water(25:75).column tempreture 25℃,and the flow rare is 1.0ml/min.The detection wavelength is 270nm.Results A better linear relationship was obtained form 0.01949～0.3898μg of polydatin in plasma,which is with a good correlation coefficient(r=0.9998).The average recovery of ephedrine hydrochloride was,99.90%,RSD was 1.11%.Conelutioa This method is simple and quick.It can be applied to quality control of Qiangjinjiangu granulas. |
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| Keywords: | HPLC lcariin Qiangjinjiangu granulas HPLC |
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