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发酵罐葡萄糖流加大规模异养培养小球藻
引用本文:梁世中,朱明军,孟海华,尹建云,陈峰.发酵罐葡萄糖流加大规模异养培养小球藻[J].华南理工大学学报(自然科学版),2000,28(12):66-70.
作者姓名:梁世中  朱明军  孟海华  尹建云  陈峰
作者单位:1. 华南理工大学,食品与生物工程学院,广东,广州,510640
2. 广东省江门生物技术开发中心,广东,江门,529081
3. 香港大学植物学系,香港薄扶林道
摘    要:应用流加工艺在5,50,200,800,4000L机械搅拌发酵罐中大规模异养培养小球藻,与间歇异养或光照自养相比,流加培养大大地提高了细胞密度和生产率,最高细胞质量浓度达到43.31g/L,比生长速率达到0.069h^-1,细胞生产率达到0.62g/(L.h),结果表明,利用传统的机械搅拌发酵罐大规模生产小球有良好的商业化前景。

关 键 词:小球藻  搅拌发酵罐  大规模异常培养  葡萄糖
修稿时间:2000-04-28

Heterotrophic Mass Cultures of Chlorella Vulgaris with Glucose Feeding in Fermenters
LIANG Shi-zhong,ZHU Ming-jun,MENG Hai-hua,YIN Jian-yun,CHEN Feng.Heterotrophic Mass Cultures of Chlorella Vulgaris with Glucose Feeding in Fermenters[J].Journal of South China University of Technology(Natural Science Edition),2000,28(12):66-70.
Authors:LIANG Shi-zhong  ZHU Ming-jun  MENG Hai-hua  YIN Jian-yun  CHEN Feng
Abstract:Chlorella vulgaris was heterotrophically cultured in 5, 50, 200, 800, and 4 000 L stirred tank fermenters with glucose feeding,which gave significant improvement in cell density and productivity compared to batch culture. The highest cell dry weight mass concentration reached 43.31 g/L.The specific growth rate was 0.069 h-1. The cell productivity was 0.62 g/(L·h). The results suggest Chlorella vulgaris can be heterotrophically produced in stirred tank fermenters for commercial use.
Keywords:Chlorella vulgaris  stirred tank fermenter  heterotrophic mass culture
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