小鼠骨髓抑制和再生过程中Sod/Gpx/Cat抗氧化酶基因的动态表达研究

 目的 研究 Sod/Gpx/Cat 抗氧化酶系列基因在小鼠骨髓抑制和再生过程中的动态表达，了解氧化应激在骨髓再生过程中的作用。 方法 制备经 5-氟尿嘧啶（5-Fu）注射后的骨髓抑制和再生 C57BL/6 小鼠模型，分别在 5-Fu注射前（第 0 天）和注射后第 3、7、11、14 天各断颈处死 6 只小鼠（分别记为 0 d 组、3 d 组、7 d 组、11 d 组、14 d 组），收集小鼠骨髓单核细胞。Trizol 试剂抽提小鼠骨髓单核细胞总 RNA，采用 Affymetrix 小鼠全基因组 cDNA 430A 芯片检测小鼠骨髓细胞 Sod/Gpx/Cat 系列基因的表达，并对检测结果进行半定量 RT-PCR 鉴定。 结果 Sod1/Sod2/Gpx1/Gpx3b/Gpx4b/Cat 在各组小鼠骨髓细胞中均有较高的表达，Sod3/Gpx2 则在 7 d 组开始表达，11 d 组和 14 d 组表达量持续减少。其中 Sod2/Gpx3b/ Gpx4b/Cat 从 0 d 组到 14 d 组均为表达量先增加后减少；Sod1/Gpx1 则为表达量先减少后增加；在各组小鼠骨髓细胞中未检测到 Gpx3a/Gpx4a/Gpx5/Gpx6 的表达。 结论 Sod/Gpx/Cat 抗氧化酶系列基因在小鼠骨髓抑制和再生过程中呈现特征性的动态变化，这种变化提示了骨髓细胞氧化应激反应过程的组织特异性和调控特定的细胞信号通路的过程。

Dynamic expression pattern of Sod/Gpx/Cat antioxidative genes during depression and regeneration of mouse bone marrow

Objective To explore the dynamic expression profile of Sod/Gpx/Cat anti-oxidative genes during the depression and regeneration of mouse bone marrow, and the effect of oxidative stress on the regeneration of bone marrow. Methods Models of bone marrow depression and regeneration were made by injecting 5-fluorouracil (5-Fu, 250mg/kg) into C57BL/6 mice. The mice were killed before and 3, 7, 11, and 14 days after the injection respectively (0 d, 3 d, 7 d, 11 d, and 14 d groups, 6 mice in each group) to collect the bone marrow-derived monocytes. Total RNA was extracted by using Trizol agent. And then the Sod/Gpx/Cat genes were detected using Affymetrix mouse cDNA 430A gene chips, and the results of detection were identified by semi-quantitative RT-PCR. Results Sod1, Sod2, Gpx1, Gpx3b, Gpx4b, and Cat were all expressed at a high level in the bone marrow cells. Sod3 and Gpx2 began to express at 7 d group, and the expression level decreased continuously in 11 d group and 14 d group. the expression levels of Sod2, Gpx3b, Gpx4b, Cat increased at first and then gradually decreased; while the expression level of Sod1 and Gpx1 were decreased at first and then increased gradually. No Gpx3a, Gpx4a, Gpx5, or Gpx6 was detected in any of the groups. Conclusions Sod/Gpx/Cat antioxidantive genes express dynamically during the depression and regeneration of mouse bone marrow, indicating that the oxidative stress, which is tissue-specified, can regulate the specific cell signal passway in the bone marrow cells.