近交系KM-HL鼠白细胞减少症模型的建立

目的 为昆明-无毛鼠(KM-HL)作为白细胞减少症模型实验用鼠提供理论依据。方法 分别对KM-HL鼠①用环磷酰胺(Cy);②用¹³⁷Cs源-γ射线全身一次性照射7.5Gy,剂量率为:0.80645Cy/min致小鼠白细胞减少症,取样对其血液、造血系统、免疫及心脏、肝脏等生理指标的变化情况进行测试研究。结果 研究表明正常KM-HL在白细胞减少症的实验中,WBC、DNA、脾脏、胸腺、心脏等一些生理指标与对照组比较都受损较重(P<0.001),但Cy组的肝脏与对照比受损较轻,无统计学意义。结论 KM-HL鼠无论用Cy还是用¹³⁷Cs源-γ射线全身一次性照射7.5Gy,其血液、造血系统、免疫及心脏、肝脏等生理指标与对照组比都有所下降(P<0.001),可做为白细胞减少症的实验用鼠。

The Establishment of Leucopenia Model on KM-HL Mice

Objective To provide theoretical foundations for KM-HL mice used as experimental mice on leucopenia model.Methods Cyclophosphamide and 137Cs source γ rays irradiated at 7.5Gy once the whole body (dose rate:0.80645Gy/min) were given on KM-HL mice to induce mice leucopenia model, samples were obtained to examine the variation of physiological indexes such as blood, hematopoietic system, immunity, heart and liver etc.Results The research shows, in normal KM-HL mice in the leucopenia experiment, some of the physiological indexes of WBC, DNA, spleen, thymus, heart shows that they are deeply injured (P<0.001), only the Cyclophospamide group's liver is lightly injured, without any statistic difference.Conclusion Either giving cyclophosphamide or ¹³⁷Cs source γ rays irradiating once all over the body, the physiological indexes such as blood, hematopoietic system, immunity, heart and liver etc of KM-HL mice are descended, compared with the control group (P<0.001), therefore it can be used as experimental mice on leucopenia.