气体信号分子硫化氢在油酸致大鼠急性肺损伤中的作用

目的:探讨胱硫脒-γ-裂解酶(cystathionine-γ-lyase,CSE)/硫化氢(hydrogen sulfide,H2S)系统在急性肺损伤(acute lunginjury,ALI)中的作用。方法:尾静脉注射油酸(oleic acid,OA)制备大鼠ALI模型为OA组,对照组注射等量生理盐水,注射OA前给予硫氢化钠(NaHS)(14μmol/kg)作为NaHS+OA组,单纯给予NaHS(14μmol/kg)作为NaHS组,分别测定血浆及肺组织H2S生成量、CSE活性、3-巯基丙酮酸转硫酶(3-mercaptopyruvate sulfurtransferase,MPST)活性、肺组织及血浆中丙二醛(MDA)、共轭二烯(Diene)键含量;并对肺部病变进行评定。结果:与对照组比较,OA组肺组织CSE、MPST活性及H2S浓度呈现下降趋势(P<0.01),血浆H2S浓度升高(P<0.01),血浆及肺组织MDA及Diene键含量升高(P<0.01);与OA组比较,NaHS+OA组血浆及肺组织MDA和Diene键含量明显下降(P<0.01)。结论:内源性CSE/H2S系统参与了OA致大鼠ALI的病理生理过程;给予外源性H2S可以减轻ALI时肺脏脂质过氧化损害。

The effect of hydrogen sulfide on oleic acid-induced acute lung injury in rats

Objective:To explore the role of endogenous cystathionine-γ-lyase(CSE)/hydrogen sulfide(H2S)pathway in oleic acid-induced acute lung injury in rats.Method:Acute lung injury was induced by injecting oleic acid(0.2 mL/kg)intravenously as OA group;NS controls were given normal saline(0.2 mL/kg);NaHS solution(14.0 μmol/kg)was injected intraperitoneally before OA(0.2 mL/kg)injection as NaHS+OA group;and the others were injected NaHS solution(14.0 μmol/kg)intraperitoneally as NaHS group.The concentration of H2S and the contents of MDA,Diene in the lung tissue and the plasma were detected;the activity of CSE and MPST were measured.Result:Compared with NS control group,in OA group the concentration of H2S and the activity of CSE and MPST in lung tissue of rats all decreased(P<0.01),The level of plasma H2S increased(P<0.01),the contents of MDA and Diene both in lung tissue and in plasma increased significantly(P<0.01);In NaHS+OA group,the contents of MDA and Diene decreased significantly(P<0.01).Conclusion:Endogenous CSE/H2S pathway was involved in the pathogenesis of acute lung injury;Exogenously applied H2S could attenuate the process of acute lung injury possibly because of reducing oxidative stress.