大黄素对大鼠原位肝移植急性排斥反应中 TNF-α，IL-10水平的影响

目的：探讨大黄素对大鼠肝移植急性排斥反应的作用机制。方法：以近交系大鼠建立原位肝移植模型，分为3组，每组各16对：BN→BN同基因对照组，Lewis→BN急性排斥组，Lewis→BN大黄素干预组。各组随机选取6只受体大鼠，于术后1，3，5，7 d的取尾静脉血样，ELISA法检测TNF-α，IL-10含量，并于第7日处死，检测病理组织学改变。其余每组各10只观察其生存状况和生存期。结果：大黄素干预组术后中位生存时间为25.6 d，较排斥组10.9 d明显延长(P<0.05)；与排斥组相比，大黄素干预组肝脏组织病理排斥反应明显降低(P<0.01)，外周血TNF-α的含量明显下降(P<0.05)，IL-10上调，自术后3 d有统计学差异(P<0.05)。结论：大黄素能够有效抑制肝移植大鼠的急性排斥反应，其作用与降低外周血TNF-α的含量，上调IL-10水平有关。

Effect of emodin on rejection of liver transplantation in rats

Objective: To investigate the mechanism of Emodin on the role of acute rejection in rat liver transplantation.  Method: Forty-eight pairs of orthotopic liver transplantation model were established with inbred rats which were randomly divided into 3 groups: Control group (BN→BN), acute rejection group (Lewis→BN) and emodin group (Lewis→BN). Six recipients in each group were randomly collected and contents of TNF-α and IL-10 in the peripheral blood were detected with ELISA on Day1, 3, 5 and 7 separately after transplantation and histopathological evaluation was made to detect the differences among groups after the livers were taken out on day7. The other 10 in each group were protected to evaluate the animation and life time.  Result: The average meso-life time in emodin group (25.6 days) is significantly longer (P<0.05) than acute rejection group (10.9 days). Compared with the acute rejection group, Emodin group shows up less rejection in the histopathological evaluation (P<0.01), less TNF-α (P<0.05) and a significant up-regulation of IL-10 in the peripheral blood (P<0.05 after day3).  Conclusion: Emodin can inhibit the acute rejection of liver transplantation in rats model effectively and it may play the role with reduction of TNF-α and upregulation of IL-10.