| 采用褪色光度法测定维生素C |
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| 引用本文： | 桑宏庆,王丽,柯林俊.采用褪色光度法测定维生素C[J].饮料工业,2010,13(11):42-45. |
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| 作者姓名： | 桑宏庆 王丽 柯林俊 |
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| 作者单位： | 安徽科技学院,安徽凤阳233100 |
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| 基金项目： | 安徽科技学院自然科学课题资助项目 |
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| 摘 要： | 在硫酸介质中,利用维生素C（L-VC）对高锰酸钾的褪色效应建立了褪色光度法测定维生素C的方法。在最大吸收波长为526nm,表观摩尔吸收系数为6.71×103L·mol-1·cm-1时,维生素C浓度在0～20μg/ml范围内与溶液的褪色程度（△A）服从比尔定律,最小检出限为0.91mg/L,相对标准偏差为0.54%～0.96%,回收率为92.5%～101.8%。
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| 关 键 词： | 维生素C 褪色光度法 测定 |
Determination of ascorbic acid by discoloration spectrophotometry |
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| SANG Hong-qing,WANG Li,Ke Lin-jun.Determination of ascorbic acid by discoloration spectrophotometry[J].Beverage Industry,2010,13(11):42-45. |
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| Authors: | SANG Hong-qing WANG Li Ke Lin-jun |
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| Affiliation: | (Anhui Science and Technology University, Fengyang 233100, Anhui, China) |
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| Abstract: | A new method of determining ascorbic acid by discoloration spectrophotometry was established by using the discoloring effect of ascorbic acid （L-VC） on potassium permanganate in sulfuric acid medium. At the maximum absorption wavelength of 526 nm and the apparent molar absorption coefficient of 6.71 ×10^3 L·mo1-1· cm-1, Beer＇s Law was obeyed in the concentration of VC ranging from 0 to 20 μg·mL-1 and the discoloration degree（ΔA）. The detection limit was 0.91 mg/L, the relative standard |
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| Keywords: | ascorbic acid discoloration spectrophotometry determination |
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