生物合成5-氨基乙酰丙酸的发酵条件

筛选出一株能合成5-氨基乙酰丙酸的紫色非硫红假单胞菌，并且对该菌株合成5-氨基乙酰丙酸的影响因素进行了详细探索。结果表明：光照厌氧为该菌的最佳培养方式，谷氨酸钠为最适碳源，酵母膏为最适氮源；通过正交实验得到谷氨酸钠最佳浓度为2 g • L－1，酵母膏最佳浓度为1.5 g • L－1；最佳接种量为20%；初始pH值为7.0。该条件下，5-氨基乙酰丙酸的产量可达到49.39 mg • L－1。

Fermentation for biosynthesis of 5-aminolevulinic acid by photosynthetic bacteria strain

A strain of rhodopseudomonas sp synthesizing 5-aminolevulinic acid was screened and the affecting factors for biosynthesis were investigated. The experiment results showed that the culture with light and no oxygen was the best culture mode for the production of 5-aminolevulinic acid. Sodium glutamate was the best carbon source and yeast extract was the best nitrogen source for the strain. According to the results of orthogonal design test，the optimal culture medium for the production of 5-aminolevulinic acid was decided. The best concentration of sodium glutamate was 2.0 g • L－1. The best concentration of yeast extract was 1.5 g • L－1. The best inoculum size was 30%，The best pH was 7.0. The experiment results showed that the production of 5-aminolevulinic acid could be increased greatly as compared with literature. Under this optimal conditions，the production of 5-aminolevulinic acid by this strain was as high as 49.39 mg • L－1.