重组人骨形态发生蛋白2与碱性成纤维细胞生长因子联合促进兔骨髓基质细胞血管内皮细胞生长因子表达及其成骨潜能

背景:了解在体外或体内环境下,应用重组人骨形态发生蛋白2和碱性成纤维细胞生长因子,能否达到既加强成骨又促进血管内皮细胞生长因子表达的双重作用。目的:观察兔骨髓基质细胞经重组人骨形态发生蛋白2与碱性成纤维细胞生长因子刺激后,其血管内皮细胞生长因子表达及成骨潜能的变化。方法:取兔双侧股骨骨髓基质细胞,采用骨髓基质细胞体外培养技术,单独或联合以重组人骨形态发生蛋白2、碱性成纤维细胞生长因子刺激细胞。细胞培养5d后,进行细胞形态、增殖情况、碱性磷酸酶活性、成骨结节、血管内皮细胞生长因子阳性细胞率等项目的检测。结果与结论:联合先后应用重组人骨形态发生蛋白2、碱性成纤维细胞生长因子在细胞计数、碱性磷酸酶活性、矿化面积百分率、血管内皮细胞生长因子阳性细胞率4个检测项目上优于同时应用重组人骨形态发生蛋白2或碱性成纤维细胞生长因子以及单独应用重组人骨形态发生蛋白2或碱性成纤维细胞生长因子。结果表明合理的联合使用重组人骨形态发生蛋白2和碱性成纤维细胞生长因子,不仅可促进骨髓基质细胞的快速增殖及向成骨细胞转化,还可促进促血管内皮细胞增生的重要介质血管内皮细胞生长因子的表达。

Recombinant human bone morphogenetic protein-2 combined with basic fibroblast growth factor promotes vascular endothelial growth factor expression and osteogenic potential of bone marrow stromal cells in rabbits

BACKGROUND:Whether recombinant human bone morphogenetic protein-2(rhBMP-2) and basic fibroblast growth factor(bFGF) can reach the dual role of the strength of osteogenesis and promotion of bFGF expression is need to know in vitro or in vivo experiment.OBJECTIVE:To investigate the effects of rhBMP-2 and bFGF on the vascular endothelial growth factor(VEGF) expression and osteogenic potential of marrow stromal cells(MSCs).METHODS:The MSCs of the femur of the rabbit were procured and cultured by MSCs cultured in vitro.The samples were treated with rhBMP-2 or(and) bFGF.After culturing for five days,the morphology of cells,proliferation,alkaline phosphatase activities(APA),bone clusters,and the ratio of VEGF-positive cells were detected.RESULTS AND CONCLUSION:The effects of combined use of BMP-2 and bFGF on cell counting,APA,percentage of mineralized area,and the ratio of VEGF-positive cells were better than that of using rhBMP-2 or bFGF singly.The results showed that a rational combined use of rhBMP-2 and bFGF can not only enhances multiplication ability and osteogenic transformation of MSCs,but also promotes VEGF expression of the proliferation of VEGF.