| RP-HPLC法测定浓维磷糖浆中咖啡因和维生素B1的含量 |
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| 引用本文： | 陈雪帆,包瑶瑶.RP-HPLC法测定浓维磷糖浆中咖啡因和维生素B1的含量[J].中国卫生检验杂志,2012(5):1016-1017,1020. |
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| 作者姓名： | 陈雪帆 包瑶瑶 |
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| 作者单位： | 浙江省食品药品检验所;浙江中医药大学 |
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| 摘 要： | 目的:采用反相高效液相色谱法测定浓维磷糖浆中咖啡因和维生素B1的含量。方法:采用Dia-monsil C18柱(250 mm×4.6 mm,5μm),以甲醇-庚烷磺酸钠缓冲液为流动相(10∶90),检测波长为272 nm。结果:咖啡因与维生素B1浓度分别在3.5μg/ml～282.6μg/ml(r=1.0000)和0.7μg/ml～54.7μg/ml(r=0.9999)内与峰面积呈良好的线性关系(n=9),方法回收率(n=9)分别为99.2%和99.1%,RSD分别为1.8%和1.7%。结论:该方法操作简便,结果准确可靠,可以有效地控制产品质量。
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| 关 键 词： | 浓维磷糖浆 咖啡因 维生素B1 反相高效液相色谱法 |
RP-HPLC determination of the content of caffeine and vitamine B1 in concentrated divitamins and sodium phosphate syrup |
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| CHEN Xue-fan,BAO Yao-yao.RP-HPLC determination of the content of caffeine and vitamine B1 in concentrated divitamins and sodium phosphate syrup[J].Chinses Journal of Health Laboratory Technology,2012(5):1016-1017,1020. |
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| Authors: | CHEN Xue-fan BAO Yao-yao |
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| Affiliation: | 1.Zhejiang Institute for Food and Drug Control,Hangzhou 310004,China;2.Zhejiang Chinese Medical University,Hangzhou 310053,China) |
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| Abstract: | Objective:To establish a method for determination of caffeine and vitamine B1 in concentrated divitamins and sodium phosphate syrup.Methods: The reversed phase HPLC system consisted of a C18 column(250 mm*4.6 mm,5 μm).The mobile phase consisted of methanol and heptanesulfonate sodium(10∶ 90),and the wavelength of detector was 272 nm.Results: The linear response range of caffeine and vitamine B1 were 3.5 μg/ml~282.6 μg/ml(r=1.0000) and 0.7 μg/ml~54.7 μg/ml(r=0.9999),respectively(n=9).The average recovery rates(n=9) of caffeine and vitamine B1 were 99.2%(RSD=1.8%) and 99.1%(RSD=1.7%),respectively.Conclusion: This method is simple and convenient in work.It is accurate and suitable for the quality control. |
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| Keywords: | Concentrated divitamins and sodium phosphate syrup Caffeine Vitamine B1 RP-HPLC |
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