类多囊卵巢综合征大鼠模型的比较研究

目的复制用雄激素及人绒毛膜促性腺激素（HCG）诱导的两种类多囊卵巢综合征（PCOS）大鼠模型，比较两种模型的差异。方法模型1组给9日龄SD雌性大鼠注射丙酸睾丸酮，模型2组给予24日龄SD雌性大鼠皮下埋植左炔诺孕酮，3d后所有模型2组大鼠皮下注射HCG9d。复制出两种类PCOS大鼠模型，分别观察两组模型的排卵情况、卵巢形态学改变、血浆雄激素（T）、雌二醇（E2）、卵泡刺激素（FSH）、促黄体生成素（LH）、空腹血糖（FPG）、空腹胰岛素（FINS）水平。结果模型1组与模型2组大鼠卵巢均成多囊性改变，卵泡囊性扩张，颗粒细胞层数减少、膜间质细胞增生；但模型1组大鼠卵巢体积较其正常对照组缩小，模型2组大鼠卵巢体积较其正常对照组增加。模型1、2组大鼠血清总T、FINS、FPG水平均明显升高；模型1组大鼠血清E2水平较其正常对照组升高，血清FSH、LH水平较其正常对照组降低；模型2组大鼠血清FSH、E2水平较其正常对照组下降，血清LH水平较其正常对照组升高。结论丙酸睾丸酮和HCG诱导的两种类型PCOS大鼠动物模型多囊卵巢、高雄激素血症、高胰岛素血症等现象均与人PCOS类似，两组虽然在某些方面与人PCOS存在一定差异，但却各自有其与人PCOS相似的特征，两者均可作为类PCOS动物模型用于不排卵的研究目的。

Comparison of Polycystic Ovary-Like Syndrome Models in Rats Established by Subcutaneous Injection of Testosterone Propionate or HCG

Objective Two polycystic-like ovary syndrome models in rats induced by testosterone or HCG were compared.Methods In the model 1,Female Sprague-Dawley rats at age of 9 days were given subcutaneous injection of testosterone propionate.In the model 2,Female Sprague-Dawley rats at age of 24 days were given subcutaneous implant of levonorgestrel.3 days later HCG was subcutaneously injected to these rats for 9 days.Then the ovulation,morphology of ovary,blood serum levels of testosterone(T),estradiol(E-2),follicular stimulating hormone(FSH),luteinzing hormone(LH),fasting plasma glucose(FPG),fasting insulin(FINS) in the two models were assyed.Results Rats of both two models showed polycystic ovary-like syndrome manifestation.But the ovary of rats in model 1 was smaller than that of controls,and bigger in model 2.Serum levels of T,FPG,FINS increased in two models.Serum levels of E-2 increased,FSH and LH decreased in model 1.Serum levels of FSH and E-2 decreased,LH increased in model 2.Conclusion The two polycystic ovary-like syndrome rat models manifested with PCO,hypertestosteronemia and hyperinsulinemia.Those rats showed similar changes in human PCOS,and can be used as model animals for certain rat experimental research.