分化相关基因1在Ⅰ型子宫内膜癌中的表达

目的]检测NDRG1基因在正常子宫内膜和Ⅰ型子宫内膜癌中的表达，探讨其在Ⅰ型子宫内膜癌发生中的作用机制。方法]应用免疫组化、原位杂交及RT—PCR技术检测NDRG1基因在正常子宫内膜与Ⅰ型子宫内膜癌中的表达。结果]免疫组化结果显示，NDRG1蛋自在正常子宫内膜和Ⅰ型子宫内膜癌中的表达率分别为12．5％和83．5％，有显著性差异（P=0．000）；原位杂交结果显示．NDRG1 mRNA在10例正常子宫内膜中只有2例表达．而在30例Ⅰ型子宫内膜癌中有22例表达，有显著性差异（P=0.007）；RT-PCR结果显示，NDRG1mRNA在Ⅰ型子宫内膜癌中的表达比正常内膜增高3倍（P=0.000）。结论]NDRG1在子宫内膜癌中在蛋白水平和mRNA水平与正常子宫内膜相比都明显升高。它可能是一种肿瘤相关基因，其异常表达可能参与了Ⅰ型子宫内膜癌的发病机制。

Expression of NDRG1 in Endometrioid Adenocarcinoma Type Ⅰ

Purpose] To explore the expression of NDRG1 in normal endometrium and endometriold adenocarcinoma type Ⅰ , and to investigate its carcinogenetic mechanism in endometrioid adenocarcinoma, Methods] The expression of NDRG1 in normal endometrium and endometrioid adenocarcinoma were examined by immunohistochemistry, in situ hybridization and RT-PCR methods. Results] The immunohistochemistry result indicated that the positive expression rate of NDRG1 protein in normal endometrium and endometrioid adenocareinoma was 12.5% and 83.5% respectively, with significant difference （P=0.000）; NDRG1 mRNA expressed in 2 of 10 cases of normal endometrium and 22 of 30 cases with endometrioid adenocarcinoma by in situ hybridization （P= 0.007）; In RT-PCR test, NDRG1 mRNA expression was 3 times higher in endometrioid adenocarcinoma than that of normal endometrium （P=0.000）. Conclusion] NDRG1 expression is upregulated significantly in endometrioid adenocarcinoma in contrast with normal endometrium both at protein and mRNA level （P〈0.01）, NDRG1 might be a tumor related gene. Its overexpression may participate in the carcinogenesis of endometrioid adenocarcinoma.