非离子表面活性剂萃取-高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚

目的：以5g/100mL非离子表面活性剂Triton X-100为萃取剂，建立一种简单快速的采用超声提取，高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚的方法。方法：采用Phenomenex C18色谱柱(250mm×4.6mm，5.0μm)，以甲醇-0.25%乙酸溶液为流动相梯度洗脱，流速1.0mL/min，紫外检测波长350nm。结果：绿原酸、槲皮素和山奈酚分别在0.011～220μg/mL(r＝0.9997)、0.065～130μg/mL(r＝0.9999)、0.068～136μg/mL(r＝0.9999)线性关系良好，检出限(RSN＝3)依次是1.94、0.22、0.27ng/mL；样品的加标回收率为90.40%～96.82%。结论：该方法具有样品前处理简单、绿色环保、灵敏快速等优点，可为枇杷叶中一些黄酮类物质和绿原酸的检测提供一个有效的方法。

Simultaneous Determination of Chlorogenic Acid,Quercetin and Kaempferol in Loquat Leaves by Non-ionic Surfactant Extraction Solvent Followed by High Performance Liquid Chromatography

Objective:An HPLC method for the simultaneous determination of chlorogenic acid,quercetin and kaempferol in loquat leaves was proposed based on rapid ultrasonic-assisted extraction using the non-ionic surfactant Triton X-100 as the extractant.Methods:The chromatographic separation was achieved on a Phenomenex C18 column using a mobile phase made up of methanol and 0.25% acetic acid at a flow rate of 1.0 mL/min,and the analytes were detected with a UV detector at 350 nm.Results:The calibration curves for chlorogenic acid,quercetin and kaempferol were linear in the range of 0.011-220μg/mL(r=0.9997),0.065-130μg/mL(r=0.9999),0.068-136μg/mL(r=0.9999),respectively.The limit of detection(LOD) was 1.94,0.22,0.27 ng/mL(RSN=3),respectively.The average recoveries across three spike levels ranged from 90.71% to 94.88%(n=3).Conclusion:The method is simple,rapid,convenient,sensitive,and environment-friendly,and thus can provide a new and scientific means for assaying the contents of flavonoids and chlorogenic acid in loquat leaves.