大孔树脂对丹参中5种丹酚酸类化合物的吸附分离特性

目的:考察8种型号大孔树脂对丹参水溶性成分中5种丹酚酸(丹参素、原儿茶醛、迷迭香酸、丹酚酸B、丹酚酸A)的吸附特性。方法:以5种丹酚酸类化合物为评价指标,采用高效液相色谱分别检测静、动态实验中各个丹酚酸类化合物的含量变化。结果:8种型号的树脂均对丹参素吸附率低。动态实验中,HP-20型树脂对迷迭香酸、丹酚酸B和丹酚酸A的动态饱和吸附量分别为30.506 mg.g^-1(干树脂),36.996 mg.g^-1(干树脂),43.85 mg.g^-1(干树脂)。结论:8种大孔树脂均不宜选用丹参素、原儿茶醛作为评价指标。

Absorption and isolation of macroporous resin for five salvianolic acids from Salviae mitiorrhizae

Objective: To study the adsorption of the macroporous resin for the five salvianolic acids(danshen su,rosmarinic acid, protocate chualdehyde,salvianolic acid B,salvianolic acid A,extracted from Salviae mitiorrhizae.Method: The five salvianolic acids were employed as an index,and the change of them in the static and dynamic absorbent was detected by HPLC,respectively.Result: HP20 resin was a suitable marcoporous resin to purify salvianolic acids.the dynamic adsorption capacity of rosmarinic acid,salvianolic acid B and salvianolic acid A was 30.506 mg·g^-1(dry resin),36.996 mg·g^-1,(dry resin),43.85 mg·g^-1(dry resin) respectively.Conclusion: It is not suitable that danshensu and protocate chualdehyde are the evaluation indexes for using 8 macroporous resins to purify salvianolic acids.