| 转基因泡桐树叶及其花粉毒性与过敏性试验 |
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| 引用本文： | 樊柏林,杜涛,付少华,田辉,宋毅,王护民,张启嫒,曹克俊.转基因泡桐树叶及其花粉毒性与过敏性试验[J].湖北预防医学杂志,2009,20(4):8-10. |
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| 作者姓名： | 樊柏林 杜涛 付少华 田辉 宋毅 王护民 张启嫒 曹克俊 |
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| 作者单位： | 湖北省疾病预防控制中心;中国科学院武汉病毒所;湖北省通山县九宫山有机茶基地; |
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| 基金项目： | 国家自然科学基金资助:30170746 |
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| 摘 要： | 目的初步探讨转入抗菌肽基因的泡桐树叶及花粉的毒性与致敏性。方法采用最大灌胃量法进行泡桐树叶的急性经口毒性试验;按照化妆品卫生规范方法进行花粉的皮肤变态反应试验;采用滴鼻和腹腔注射途径给予豚鼠花粉,观察致过敏性哮喘反应。结果转基因泡桐树（树叶）对SPF级Wistar雌性和雄性大鼠急性经口LD50均〉10000mg/kg·BW;转基因泡桐树（花粉）对普通级白毛豚鼠皮肤变态反应积分为0,致敏率为0%,致敏强度为弱,未见皮肤变态反应;转基因泡桐树花粉对豚鼠未见明显过敏性哮喘反应。结论转入抗菌肽基因泡桐树叶属实际无毒级,花粉对豚鼠皮肤致敏性与过敏性哮喘反应均为阴性。
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| 关 键 词： | 转基因泡桐树 过敏性 毒性 |
Toxicity and sensitization of antibiotic peptide transgenic kiri leaf and pollen |
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| FAN Bo-lin,FU Shao-hua,TIAN Hui,SONG Yi,WANG Hu-min,ZHANG Qi-ai. Hubei Provincial Center of Disease Control , Prevention,Wuhan ,China,DU Tao. Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan ,CAO Ke-jun. Hubei Provincial Tongshan County Jiugongshan Organic Tea Advisor,Tongshan.Toxicity and sensitization of antibiotic peptide transgenic kiri leaf and pollen[J].Hubei Journal of Preventive Medicine,2009,20(4):8-10. |
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| Authors: | FAN Bo-lin FU Shao-hua TIAN Hui SONG Yi WANG Hu-min ZHANG Qi-ai Hubei Provincial Center of Disease Control Prevention Wuhan China DU Tao Wuhan Institute of Virology Chinese Academy of Sciences Wuhan CAO Ke-jun Hubei Provincial Tongshan County Jiugongshan Organic Tea Advisor Tongshan |
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| Affiliation: | FAN Bo-lin,FU Shao-hua,TIAN Hui,SONG Yi,WANG Hu-min,ZHANG Qi-ai. Hubei Provincial Center of Disease Control , Prevention,Wuhan 430079,China,DU Tao. Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,CAO Ke-jun. Hubei Provincial Tongshan County Jiugongshan Organic Tea Advisor,Tongshan 437600 |
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| Abstract: | Objective To study the acute toxicity and sensitization of antibiotic peptide transgenic kiri leaf and pollen.Methods Maximal oral dosage was administered to mice in acute oral toxicity test;Transgenic kiri pollen skin sensitization test was conducted in accordance with the method in hygienic standard for cosmetics;Transgenic kiri pollen irritable asthma test was conducted in guinea pig through nose and abdomen.Results LD50 of kiri leaf was more than 10000 mg/kg.BW in SPF rats acute oral toxicity test. Skin... |
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| Keywords: | Transgenic kiri Sensitization Toxicity |
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