Functional mapping of the activity of the R region in the human T-cell leukemia virus type I long terminal repeat to increase gene expression |
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Authors: | Masataka Nakamura Kiyoshi Ohtani Yorio Hinuma Kazuo Sugamura |
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Affiliation: | (1) Department of Bacteriology, Tohoku University School of Medicine, 980 Sendai, Japan;(2) Shionogi Institute for Medical Science, 566 Settsu-shi, Osaka, Japan |
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Abstract: | We previously demonstrated the activity of the R fragment in the long terminal repeat of human T-cell leukemia virus type I for elevation of the level of gene expression. In this study, the fragment was deleted with BAL31 nuclease to determine its functional domain. Series of the shortened R fragments were linked to the simian virus 40 promoter unit, which regulated expression of a reporter gene. Examination with the R fragments deleted from the 5 and 3 ends showed that borders of the functional domain were mapped within nucleotide positions 458 to 473 for the 5 end and nucleotide positions 559 to 594 for the 3 end, respectively. Thus we conclude that a 136-base-pair fragment corresponding to the second half of the R region was sufficient to allow elevation of the level of gene expression. |
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Keywords: | BAL31 nuclease CAT assay gene expression HTLV-I R region 5 untranslated sequence" target="_blank">gif" alt="prime" align="BASELINE" BORDER="0"> untranslated sequence |
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