| CD133免疫磁珠分选脑肿瘤干细胞及其生物学特性 |
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| 引用本文： | 齐玲,金宏,丁丽娟,温娜,唐泽波,狄阳,李蕴潜.CD133免疫磁珠分选脑肿瘤干细胞及其生物学特性[J].吉林大学学报(医学版),2011,37(3):441-444. |
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| 作者姓名： | 齐玲 金宏 丁丽娟 温娜 唐泽波 狄阳 李蕴潜 |
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| 作者单位： | 吉林医药学院病理学教研室,吉林,吉林,132013;吉林大学第一医院神经外科,吉林,长春,130021 |
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| 基金项目： | 吉林省科技厅自然科学基金资助课题(202015242); 吉林省教育厅科研基金资助课题(吉教科合字[2011]第281号,吉教科合字[2010]第532号) |
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| 摘 要： | 目的:从人脑肿瘤组织中分离、培养、纯化和鉴定脑肿瘤干细胞(BTSCs),探讨CD133免疫磁珠分选方法获取BTSCs的可行性及BTSCs的生物学特性.方法:留取人脑胶质母细胞瘤新鲜标本,分离获得脑肿瘤细胞.应用CD133免疫磁珠分选方法纯化BTSCs;流式细胞仪检测分选阳性率;神经球计数法分析CD133+/-亚群细胞神...
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| 关 键 词： | 脑肿瘤干细胞 神经干细胞 CD133 神经巢蛋白 |
| 收稿时间： | 2011-01-18 |
CD133 magnetic sorting and biological characteristics of brain tumor stem cells |
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| QI Ling,JIN Hong,DING Li-juan,WEN Na,TANG Ze-bo,DI Yang,LI Yun-qian.CD133 magnetic sorting and biological characteristics of brain tumor stem cells[J].Journal of Jilin University: Med Ed,2011,37(3):441-444. |
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| Authors: | QI Ling JIN Hong DING Li-juan WEN Na TANG Ze-bo DI Yang LI Yun-qian |
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| Affiliation: | 1.Department of Pathology,Jilin Medical College,Jilin 132013,China;2.Department of Neurosurgery, |First Hospital,Jilin University,Changchun 130021,China |
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| Abstract: | Objective To isolate and culture brain tumor stem cells(BTSCs),and explore the feasibility for obtaining BTSCs by CD133 magnetic sorting and the biological characteristics of BTSCs.Methods The fresh glioblastoma sample was dissociated,the cells were cultivated in serum-free media with growth factor,the BTSCs were purified by CD133 magnetic sorting,the positive rate of CD133+/-subgroup cells was detected by flow cytometry;neurosphere forming essay was used to analyze the self-renewal and proliferative capaci... |
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| Keywords: | brain tumor stem cells neural stem cells CD133 nestin |
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