钾离子通道Slo及其调节亚基LRRC mRNA在不同活力男性精子中的水平差异

目的探讨电压调控的钾离子(K~+)通道Slo1、Slo3与其γ调节亚基LRRC26、LRRC52在不同活力男性精子中的mRNA水平。方法收集精液参数正常者与特发性弱精子症患者的精子提取物,采用实时荧光定量PCR技术测定电压调控的K~+通道Slo1、Slo3和其γ调节亚基LRRC26、LRRC52 mRNA相对表达量,并分析其与精子活力的相关性。结果 Slo1、LRRC26、LRRC52 mRNA在特发性弱精子症患者精子中的表达水平(0.47±0.14,0.56±0.08,0.55±0.14)明显低于参数正常男性(0.99±0.21,1.16±0.24,1.00±0.21),差异均具有统计学意义(P0.05);Slo3的表达在参数正常男性精子中(1.03±0.18)亦高于特发性弱精子症者(0.71±0.19),但差异无统计学意义(P0.05)。结论电压调控的K~+通道中Slo1与γ调节亚基LRRC26、LRRC52 mRNA的低表达与精子活力低下相关。

The levels of potassium channel Slo and the γ regulating subunit LRRC mRNA in the human sperm with different motility

Objective To explore levels ofpotassium channel Slo (Slo 1 and S1o3) and their γ regulating subunit (LRRC26 and LRRC52) in human sperm,and their relationship with sperm motility.Methods Collected semen samples from men with asthenospermia or normal semen parameters,relative mRNA expression of Slo1,Slo3,LRRC26 and LRRC52 were detected by real-time qPCR,and its association with sperm motility was analysised.Results Slo1,LRRC26 and LRRC52 mRNA were statistically lowly expressed in sperm of asthenospermia patients (0.47±0.14,0.56±0.08,0.55± 0.14),compared with that of normal male (0.99±0.21,1.16±0.24,1.00± 0.21),the expression of S1o3 in asthenospermia patients is also decreased,but the difference was not significant (0.71 ± 0.19,1.03± 0.18;P＞0.05).Conclusion The low expression of Electric volume regulated Slo 1 and its γ regulating subunit (LRRC26 and LRRC52 mRNA) are related with poor sperm motility in human.