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| 中华结缕草(Zoysia sinica Hance)组织培养和再生植株研究 | |
| 引用本文： | 韦善君,孙振元,钱永强,余龙江,韩蕾.中华结缕草(Zoysia sinica Hance)组织培养和再生植株研究[J].武汉植物学研究,2004,22(5):455-458. |
| 作者姓名： | 韦善君 孙振元 钱永强 余龙江 韩蕾 |
| 作者单位： | 1. 华中科技大学生命科学与技术学院,武汉,430074 2. 中国林业科学院林业研究所,北京,100091 3. 北京林业大学,北京,100083 |
| 基金项目： | 国家"863"项目资助(2002AA241061)。 |
| 摘 要： | 以中华结缕草(Zoysia sinica Hance)成熟种子为外植体在附加2．5mg／L2,4-D、0．25mg／L 6-BA和1～2mg／L VB1的改良MS培养基(MSm)上愈伤组织的诱导率最高为43．0％。愈伤组织的最佳继代培养基为MSm附加0．1mg／L 6-BA和2．0mg／L 2,4-D。在无生长调节物质的MS培养基(MS0)上,外观呈白色到淡黄色、含有密实颗粒的愈伤组织再生率为30％～60％。 |
| 关 键 词： | 中华结缕草 成熟胚 愈伤组织 植株再生 |
| 文章编号： | 1000-470X(2004)05-0455-04 |
Plant Regeneration from Mature Embryo of Zoysia sinica Hance in vitro |
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| WEI Shan-Jun,SUN Zhen-Yuan.Plant Regeneration from Mature Embryo of Zoysia sinica Hance in vitro[J].Journal of Wuhan Botanical Research,2004,22(5):455-458. | |
| Authors: | WEI Shan-Jun SUN Zhen-Yuan |
| Affiliation: | WEI Shan-Jun~1,SUN Zhen-Yuan~ |
| Abstract: | Mature seeds were used as explant to study the callus induction, subculture, and plant regeneration of Zoysia sinica Hance. The results showed that the maximum rate of callus induction was 43.0% and occurred on the modified MS medium (MS_m) supplemented with 2.5 mg/L 2,4-D, 0.25 mg/L 6-BA, and 1-2 mg/L thiamine. The optimum subculture medium was MS_m containing 0.1 mg/L 6-BA and 2.0 mg/L 2,4-D. 30%-60% calli type of white to yellowish could regenerate plants on MS medium free of growth regulators. |
| Keywords: | Zoysia sinica Hance Mature embryo Callus Plant regeneration |
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