| 疟疾的实时荧光PCR快速检测方法 |
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| 引用本文： | 师永霞,苏锦坤,洪烨,李小波,郑夔,黄吉城,幸芦琴,相大鹏,郭波旋.疟疾的实时荧光PCR快速检测方法[J].中国卫生检验杂志,2011(3):625-627. |
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| 作者姓名： | 师永霞 苏锦坤 洪烨 李小波 郑夔 黄吉城 幸芦琴 相大鹏 郭波旋 |
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| 作者单位： | 广东出入境检验检疫局检验检疫技术中心; |
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| 基金项目： | 广东检验检疫局科技计划项目(2007GDK32) |
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| 摘 要： | 目的:建立疟疾的实时荧光PCR检测方法并用于疟疾的快速检测。方法:设计了疟原虫通用型实时荧光PCR检测的引物和探针,建立了疟疾的实时荧光PCR检测方法。对40份疟疾镜检阳性样本和20份镜检阴性样本进行实时荧光PCR检测,并和巢式PCR方法检测结果进行了比较。结果:疟疾实时荧光PCR检测速度快,20分钟即可完成。40份镜检阳性样本的荧光PCR检测均为阳性,2份样本的巢式PCR结果为阴性;20份镜检阴性样本中有3份样本为实时荧光PCR阳性,其余17份样本为实时荧光PCR阴性,与疟疾巢式PCR检测结果相同。结论:该实时荧光PCR方法检测速度快,特异性强,准确性高,阳性率高,不易漏检,适用于疟疾的快速检验,这对防止该病在国境口岸的传入提供了技术依据。
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| 关 键 词： | 疟疾 小亚单位核糖体核糖核酸 实时荧光PCR 巢式PCR |
Real-time fluorescent PCR method for rapid detection of malaria |
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| SHI Yong-xia,SU Jin-kun,HONG Ye,LI Xiao-bo,ZHENG Kui,HUANG Ji-cheng,XING Lu-qin,XIANG Da-peng,GUO Bo-xuan.Real-time fluorescent PCR method for rapid detection of malaria[J].Chinses Journal of Health Laboratory Technology,2011(3):625-627. |
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| Authors: | SHI Yong-xia SU Jin-kun HONG Ye LI Xiao-bo ZHENG Kui HUANG Ji-cheng XING Lu-qin XIANG Da-peng GUO Bo-xuan |
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| Affiliation: | SHI Yong-xia,SU Jin-kun,HONG Ye,LI Xiao-bo,ZHENG Kui,HUANG Ji-cheng,XING Lu-qin,XIANG Da-peng,GUO Bo-xuan (Health Quarantine Lab,Guangdong Inspection and Quarantine Technology Center,Guangdong Entry-Exit Inspection and Quarantine Bureau,Guangzhou 510700,China) |
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| Abstract: | Objective:To establish real-time fluorescent PCR detection method for rapid detection of malaria.Methods:Universal primers and probes were designed.Forty positive samples and twenty negative samples tested by microscopic examination were detected Plasmodium by real-time fluorescent PCR.Their results were compared with that of nested PCR.Results:Real-time fluorescence PCR detection of malaria was fast and the reaction time only took 20 minutes.All of forty microscopy-positive samples were positive for fluore... |
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| Keywords: | Malaria SSU rRNA Real-time fluorescent PCR Nested PCR |
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