A new chrysanthemum potyvirus: molecular evidence |
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| Authors: | A Mehra N Jabeen A K Singh V Hallan |
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| Affiliation: | Plant Virology Laboratory, Floriculture Division , Institute of Himalayan Bioresource Technology, (CSIR) , Palampur, Himachal Pradesh, 176061, India |
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| Abstract: | Abstract A number of viruses are known to infect chrysanthemum plants, however in the present study a previously unknown potyvirus was detected using techniques such as ELISA, RT-PCR and hybridization. The ELISA-positive samples were amplified using a potyvirus group-specific primer which gave an amplification of ～850 bp. The amplified product was cloned and sequenced, and shows 72 – 73% homology with known potyviruses that infect chrysanthemums such as Potato virus Y potyvirus, Soyabean mosaic virus and Turnip mosaic potyvirus when compared to the sequence available in the database. However, present potyvirus isolates show 93% homology with Chilli veinal mottle virus and Pepper vein banding virus. The results were further confirmed by Northern hybridization. This is the first report of a potyvirus similar to Chilli veinal mottle virus, and Pepper vein banding virus infecting chrysanthemums. |
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| Keywords: | Chrysanthemum potyvirus RT-PCR ELISA sequencing northern hybridization |
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