沙地云杉生态型同工酶研究

沙地云杉是内蒙古地区特有树种,只分布于浑善达克沙地东部边缘;由于长期适应干旱生态条件,分化出3种土壤生态型,即紫果型、红果型和绿果型沙地云杉,并在很多生态、生理特征上表现出明显的差异.本文采用聚丙烯酰胺凝胶电泳技术,分析了3种生态型沙地云杉的过氧化物同工酶和酯酶同工酶的酶谱特征.结果表明:不管是过氧化物同工酶还是酯酶同工酶,紫果型沙地云杉的酶带条数最多,红果型其次,绿果型酶带条数最少.在酶带强度方面也有类似的结果,紫果型沙地云杉具有较多的强、次强、中带,红果型其次,绿果型最少.这在一定程度上表明,在干旱条件下产生的3种不同生态型沙地云杉,以紫果型对干旱抗性最强,绿果型最弱,红果型居于二者之间,这一结论将为沙地云杉的经营管理及品种选择提供科学依据. 图2参21

ISOZYME OF DIFFERENT ECOTYPES OF PICEA MONGOLICA

Picea mongolica W. D. Xu is an endemic tree species in Inner Mongolia Autonomous Region, China, and it is found only at eastern edge of the Hunshandake sandy land and with three ecotypes, that is, f. purpurea f. nov , f. rubra f. nov and f. viridis f. nov , due to its adaptation to drought for a long time. At the same time, the three ecotypes are quite different in ecological and physiological characters. Based on PAGE, zymogram of peroxidase and lipase isozyme of the three ecotypes P. mongolica were studied, and the result showed that no matter what peroxidase isozyme or lipase isozyme was, there were the same phenomena. f. purpurea f. nov Picea mongolica possessed most enzyme belts, then the f. rubra f. nov and the f. viridis f. nov had lest enzyme belts. As far as the intensity of enzyme belts were concerned, the f. purpurea f. nov had more strong, secondarily strong, and medium enzyme belts, and the f. viridis f. nov had fewer. It proved that the f. purpurea f. nov had the strongest drought resistance, and the f. viridis f. nov had the lowest. The conclusion could be applied as scientific basis for management and selection of P. mongolica. Fig 2, Ref 21