| 荧光脂质体法研究芍药甘草汤对磷脂酶A2的抑制效应及其配伍作用 |
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| 引用本文： | 刘陶世,赵新慧,段金廒,狄留庆,黄耀洲.荧光脂质体法研究芍药甘草汤对磷脂酶A2的抑制效应及其配伍作用[J].中草药,2008,39(7):1000-1003. |
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| 作者姓名： | 刘陶世 赵新慧 段金廒 狄留庆 黄耀洲 |
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| 作者单位： | 刘陶世 (南京中医药大学江苏省方剂研究重点实验室,江苏,南京,210029)； 赵新慧 (南京中医药大学江苏省方剂研究重点实验室,江苏,南京,210029)； 段金廒 (南京中医药大学江苏省方剂研究重点实验室,江苏,南京,210029)； 狄留庆 (南京中医药大学江苏省方剂研究重点实验室,江苏,南京,210029)； 黄耀洲 (南京中医药大学江苏省方剂研究重点实验室,江苏,南京,210029)； |
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| 基金项目： | 江苏省科技厅自然科学基金，江苏省方剂研究重点实验室开放课题 |
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| 摘 要： | 目的 采用荧光脂质体研究芍药甘草汤对磷脂酶A2(PLA2)的抑制效应及其配伍作用.方法 以磷脂酰胆碱和胆固醇等为主要材料,采用薄膜分散法-超声-膜挤出-葡聚糖凝胶柱色谱分离-冷冻干燥的联用技术制备包封羧基荧光素的脂质体;利用PLA2能水解磷脂破坏脂质体造成羧基荧光素渗漏的原理,将药物、PLA2和荧光脂质体共同温孵,检测从脂质体渗漏的羧基荧光索的荧光强度,该强度反映药物对PLA2的抑制强度.结果 芍药甘草汤、甘草、甘草酸和对照药氯丙嗪对PLA2活性均具有较强的抑制作用,白芍抑制作用较弱,而芍药苷几乎无抑制作用;芍药甘草汤复方对PLA2的抑制作用强于单味甘草和白芍.结论 芍药甘草汤对PLA2具有较强的抑制活性,白芍与甘草具有协同作用.
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| 关 键 词： | 芍药甘草汤 甘草酸 磷脂酶A2(PLA2) 脂质体 |
| 收稿时间： | 2007/10/8 0:00:00 |
Inhibition and compatibility of Shaoyao Gancao Decoction on phospholipase A2 by fluorescent liposome |
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| LIU Tao-shi,ZHAO Xin-hui,DUAN Jin-ao,DI Liu-qing and HUANG Yao-zhou.Inhibition and compatibility of Shaoyao Gancao Decoction on phospholipase A2 by fluorescent liposome[J].Chinese Traditional and Herbal Drugs,2008,39(7):1000-1003. |
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| Authors: | LIU Tao-shi ZHAO Xin-hui DUAN Jin-ao DI Liu-qing and HUANG Yao-zhou |
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| Affiliation: | Jiangsu Key Laboratory for Research of Pharmacology of Traditional Chinese Medical Formula, Nanjing Universityof Traditional Chinese Medicine, Nanjing 210029, China;Jiangsu Key Laboratory for Research of Pharmacology of Traditional Chinese Medical Formula, Nanjing Universityof Traditional Chinese Medicine, Nanjing 210029, China;Jiangsu Key Laboratory for Research of Pharmacology of Traditional Chinese Medical Formula, Nanjing Universityof Traditional Chinese Medicine, Nanjing 210029, China;Jiangsu Key Laboratory for Research of Pharmacology of Traditional Chinese Medical Formula, Nanjing Universityof Traditional Chinese Medicine, Nanjing 210029, China;Jiangsu Key Laboratory for Research of Pharmacology of Traditional Chinese Medical Formula, Nanjing Universityof Traditional Chinese Medicine, Nanjing 210029, China |
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| Abstract: | Objective To study the inhibition and compatbility of Shaoyao Gancao Decoction on phospholipase A2 (PLA2) by fluorescent liposome.Methods Liposome encapsulated carboxyfluorescein was prepared with phosphtidyl choline and cholesterol by conventional rotary-evaporated film dispersion, sonication, membrane extrusion, and purified on Sephadex G-50, then lyophilized. The drug, PLA2, and fluorescent liposome were incubated. The content of the leakage carboxyfluorescein from liposome was determined by fluorescence spectrophotometry. The content indicated the inhibition degree of drug on PLA2. Results The inhibition of Shaoyao Gancao Decoction, Glycyrrhiza uralensis, glycyrrhizin, and chlorpromazine on PLA2 respectively was strong, while the inhibition of Paeoniae Alba was weak and paeoniflorin had little inhibition. Conclusion The inhibition of Shaoyao Gancao Decoction on PLA2 is strong. Glycyrrhiza uralensis and Paeoniae Alba show the synergism in inhibiting PLA2. |
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| Keywords: | Shaoyao Gancao Decoction glycyrrhizin phospholipase A2(PLA2) liposome |
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