锰对小鼠生育指数和生精上皮细胞数影响

目的研究锰对雄性小鼠生殖能力的影响，并探讨其时问一效应和剂量一效应关系。方法将雄性小鼠随机分为3个染锰组和1个对照组，分别给予腹腔注射7．5，15．0，30．0me,／kg氯化锰和生理盐水，于染锰第3，7，14，28，56d处死小鼠5只／组。观察小鼠睾丸脏器系数和染锰56d各组雄性小鼠生殖能力、曲细精管横截面积及生精上皮细胞数量。结果染锰56d，与对照组比较，15和30mg／kg组睾丸脏器系数差异有统计学意义（P〈0．05，P〈0．01）；30mg/kg组生育指数和总死胎率差异有统计学意义（P〈0．05，P〈0．05）；3个染锰组的平均着床数与平均活胎率差异均有统计学意义（P〈0．01）；曲细精管横截面积和生精上皮细胞数差异均有统计学意义（P〈0．01）。结论各剂量的MnCl2对雄性小鼠生殖能力均有损害作用，以染锰56d，30mg／kg对雄性小鼠生殖能力的损害最为严重。

Effects of manganese chloride on exponent of birth and quantity of seminiferous epithelium cell of mice

Objective To study the effects of manganese chloride on reproductive function of male mice,and explore the dose-effects,and time-effects.Methods The mice were randomly divided into three treatment groups with manganese chloride(7.5,15.0,30.0 mg/kg) and 1 control group with NS.To observe the weight coefficient of testis after 5 different periods (3,7,14,28,56d) i.p.administration MnCl2(7.5,15.0,30.0mg/kg),the reproductive ability of male mice,changes of cross section area of seminiferous tubule and quantity of seminiferous epithelium cell were investigated after 56 d of manganese exposure.Results After 56-day manganese exposure,the weight coefficient of testis in 15,30 mg/kg groups had distinctive difference as compared with control group(p<0.05,p<0.01);the exponent of birth and the radio of dead fetus in 30 mg/kg group had significant difference as compared with control group(p<0.05,p<0.05);and the average of live fetus and ratio of nidation in 3 manganese exposure groups had distinctive difference as compared with control group(p<0.01);cross section area of seminiferous tubule and quantity of seminiferous epithelium cell as compared with control group;the difference had statistical significance(p<0.01).Conclusion The reproductive ability of male mice,it was the most serious in 30 mg/kg group after 56-day manganese exposure.