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一株耐辐射菌的分离与初步鉴定
引用本文:张志东,茆军,王玮,唐琦勇,石玉瑚.一株耐辐射菌的分离与初步鉴定[J].辐射研究与辐射工艺学报,2008,26(1):43-46.
作者姓名:张志东  茆军  王玮  唐琦勇  石玉瑚
作者单位:新疆农业科学院微生物研究所,乌鲁木齐,830000
基金项目:国家高技术研究发展计划(863计划) , 国家微生物资源平台项目 , 国家自然科学基金
摘    要:对新疆古尔班通古特沙漠土样中分离到的一株耐辐射菌RL2进行了多相分类鉴定.结果发现,此菌为革兰氏阳性,球形,菌落为淡红色;菌株的(G C)mol%含量为71.62%;16S rDNA序列分析表明,菌株RL2的16S rDNA基因序列与D.radiodurans DSM20539T同源性最高(97.2%).通过表型及系统进化树分析,确定RL2菌种分类应归于Deinococcus菌属,并可能是该菌属中的-个新种.

关 键 词:耐辐射菌  分离  鉴定  耐辐射  分离  strain  novel  identification  菌属  菌种分类  系统进化树分析  同源性  radiodurans  基因序列  分析表  rDNA  含量  菌株  菌落  阳性  革兰氏  发现  结果
收稿时间:2007-07-06
修稿时间:2007-08-21

Isolation and identification of a novel radio-resistant strain
ZHANG Zhidong,MAO Jun,WANG Wei,TANG Qiyong,SHI Yuhu.Isolation and identification of a novel radio-resistant strain[J].Journal of Radiation Research and Radiation Processing,2008,26(1):43-46.
Authors:ZHANG Zhidong  MAO Jun  WANG Wei  TANG Qiyong  SHI Yuhu
Abstract:A novel radio-resistant strain named RL2 was studied polyphasically, which was isolated from the soils in the Gurban-Tunggut Desert, Xinjiang. The strain is Gam-positive, sphere-shaped and pink pigmented; The DNA (G C) contents of RL2 is 71.62mol%; The 16S rDNA genes of RL2 and D.radiodurans type strain DSM20539 shows a high level of similarity (97.2%). According to phenotypic characteristics and phylogenetic analysis, it can be suggested that the strain RL2 has been identified as Deinococcus.sp and it may be a novel species.
Keywords:Radio-resistant strain  Isolated  Identify
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