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| miRNA-204在胃远端腺癌中的表达及作用初探 | |
| 引用本文： | 张文辉,桂俊豪,王昌正,常青,吴本俨.miRNA-204在胃远端腺癌中的表达及作用初探[J].军医进修学院学报,2011,32(7):743-747. |
| 作者姓名： | 张文辉 桂俊豪 王昌正 常青 吴本俨 |
| 作者单位： | 1. 解放军302医院肝硬化中心胃镜室,北京,100039;解放军总医院南楼消化科,北京,100853 2. 解放军总医院生化科,北京,100853 3. 解放军总医院南楼消化科,北京,100853 |
| 基金项目： | 国家科技支撑计划项目(2009BAI86B05)~~ |
| 摘 要： | 目的 筛选胃远端腺癌中差异表达的miRNAs,并就其在胃癌发生、发展中的作用及机制进行初步研究.方法 采用miRNA芯片,对胃远端(体、窦)腺癌组织及配对正常组织进行检测,筛选胃远端腺癌差异表达的miRNAs,并观察其与临床病理因素的关系;运用TargetScan等生物信息学方法,预测特定miRNA可能的靶基因.结果 芯片检测发现共47条miRNAs在胃远端腺癌组织和配对正常组织中表达差异显著;实时荧光定量PCR验证了miR-204的表达在肿瘤组织中下调;生物信息学分析显示BCL-2、NR3C1和SOCS6等可能为miR-204的靶基因.结论 胃远端腺癌有其独特的miRNA表达谱;预测BCL-2,NR3C1和SOCS6等可能为miR-204的靶基因. |
| 关 键 词： | 微小RNA 胃远端腺癌 表达谱 |
Expression of miRNA-204 in distal gastric adenocarcinoma and its role in pathogenesis and progress of gastric cancer |
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| ZHANG Wen-hui , GUI Jun-hao , WANG Chang-zheng , CHANG Qing , WU Ben-yan.Expression of miRNA-204 in distal gastric adenocarcinoma and its role in pathogenesis and progress of gastric cancer[J].Academic Journal of Pla Postgraduate Medical School,2011,32(7):743-747. | |
| Authors: | ZHANG Wen-hui GUI Jun-hao WANG Chang-zheng CHANG Qing WU Ben-yan |
| Affiliation: | ZHANG Wen-hui1,2,GUI Jun-hao3,WANG Chang-zheng2,CHANG Qing2,WU Ben-yan2 1Department of Endoscopy,Center of Liver Cirrhosis,Chinese PLA 302 Hospital,Beijing 100039,China,2Department of Geriatric Gastroenterology,Chinese PLA General Hospital,Beijing 100853,3Department of Clinical Biochemistry |
| Abstract: | Objective To study the differentially expressed miRNA-204 in distal gastric adenocarcinoma and its role in pathogenesis and progress of gastric cancer.Methods Differentially expressed miRNA-204 in distal gastric adenocarcinoma tissue and matched normal tissue was detected using miRNA microarray.Target genes of specific miRNA were predicted with bioinformatics methods such as TargetScan.Results miRNA microarray showed that the expression of miRNA-204 was significantly different in distal gastric adenocarcino... |
| Keywords: | microRNA Distal Gastric Adenocarcinoma Expression Profile |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
