| HPLC法测定枸杞子中甜菜碱的含量 |
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| 引用本文： | 方丽,祝明,郑成.HPLC法测定枸杞子中甜菜碱的含量[J].中国药品标准,2011,12(4):288-291. |
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| 作者姓名： | 方丽 祝明 郑成 |
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| 作者单位： | 1. 浙江中医药大学药学院,杭州,310053
2. 浙江省食品药品检验所,杭州,310004 |
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| 摘 要： | 目的:建立枸杞药材中甜菜碱的含量测定方法。方法:采用高效液相色谱法,色谱柱为NUCLEODUR Hilic(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%三乙胺(用磷酸调pH至7)(82:18),流速0.6 mL.min-1,检测波长196 nm。结果:甜菜碱在1.737 2～34.744μg范围内线性关系良好,r=0.999 9,平均回收率为99.5%,RSD为1.5%。结论:该方法灵敏度高,结果准确,可作为枸杞子中甜菜碱的定量测定方法。
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| 关 键 词： | 枸杞子 高效液相色谱法 甜菜碱 含量测定 |
Determination of Betaine in Lycium Chinense by HPLC |
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| Authors: | Fang Li Zhu Ming and Zheng cheng |
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| Affiliation: | Fang Li1,Zhu Ming2,Zheng cheng2(1.College of pharmacy,Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053,2.Zhejiang Institute for Food and Drug Control,Hangzhou 310004) |
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| Abstract: | Objective： To establish the quantitative method of determining the contents of betaine in lycium chinense. Methods： The chromatographic column was NUCLEODUR Hilic （4. 6 mm × 250 mm ,5 μm）. The mobile phase was aeetonitrile-0. 1% triethylamine , flow rate： 0. 6 mL · min^-1 ; column temperature： at 25 ℃ ,and UV detection was performed at 196 nm. Result： The linear range was 1. 7372 -34. 744 μg, the average recovery was 99. 5%, and RSD was 1.5% （ n =6）. Condufion： This method is accurate, re- liable as well as specific and can be used to determine the content of betaine in lycium ehinense. |
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| Keywords: | lycium chinense betaine determination HPLC |
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