抱茎獐牙菜中3种药效成分的HPLC测定

目的]测定抱茎獐牙菜中不同部位的药效成分。方法]采用反相高效液相色谱法，测定抱茎獐牙菜中不同部位獐牙菜苦苷、龙胆苦苷及芒果苷的含量。结果]结果表明，HPLC法测定3种药效成分的最佳条件为流动相甲醇-0．02％磷酸水溶液，梯度洗脱，洗脱条件0～25min，甲醇：20％～50％；流速1ml／min，检测波长为260nm。该方法具有很好的线性关系和回收率。结论]抱茎獐牙莱根部龙胆苦苷的含量最高，其余2种药效成分在花的部位有较高的含量。

Determination of Three Medicinal Bioactive Components of Swertia franchetiana H.Smith

Objective] The purpose was to determine three medicinal bioactive components in different parts of Swertia franchetiana H.Smith.Method]The content of swertiamarin,gentiopicroside and mangiferin in Swertia franchetiana H.Smith were determined by reversed-phase high performance liquid chromatography.Result]The optimum conditions of determining three medicinal bioactive components in different parts of Swertia franchetiana H.Smith were as follow: a Kromasil C18(250 mm×4.60 mm,5 μm);the mobile phase was composed of CH3OH and 0.02% phosphoric acid in water;the gradient condition was 0-25 min,CH3OH 20%-50%;the flow rate was 1 ml/min;the detection wavelength was 260 nm.The good linear relationships and recoveries were obtained under the optimum conditions.Conclusion]The content of gentiopicroside is most high in the root,swertiamarin and mangiferin have higher content in the floral part of Swertia franchetiana H.Smith.