| 直接利用糖质原料生产L-苹果酸菌种的选育 |
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| 引用本文： | 刘建军,赵祥颖,田延军,李丕武,姜鲁燕,张家祥.直接利用糖质原料生产L-苹果酸菌种的选育[J].工业微生物,2003,33(1):27-30. |
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| 作者姓名： | 刘建军 赵祥颖 田延军 李丕武 姜鲁燕 张家祥 |
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| 作者单位： | 1. 天津轻工业学院,天津,300222;山东省食品发酵工程重点实验室,济南,250013
2. 山东省食品发酵工程重点实验室,济南,250013 |
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| 基金项目： | 山东省科技攻关计划课题 :鲁科计字 ( 99) 2 5 3 |
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| 摘 要： | 从土壤中筛选到一株产L－苹果酸的黄曲霉菌，经紫外线、亚硝基胍（NTG）、硫酸二乙酯（DES）和^60Co辐射等诱变处理，并经多次分离得到一株性能稳定的高产L－苹果酸突变株黄曲霉HA5800，35℃,200r/min摇瓶发酵120h，产L－苹果酸72g/L、糖酸转化率为74．22％，经山东省卫生防疫站检定，该菌株不产黄曲霉素素B1。
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| 关 键 词： | 糖质原料 生产 L－苹果酸 黄曲霉素 菌种选育 |
Screening of over-producing L-malic acid mutant by sugar fermentation |
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| LIU Jian-jun ,ZHAO Xiang-ying ,TIAN Yan-jun ,LI Pi-wu ,JIANG Lu-yan ,ZHANG Jia-xiang.Screening of over-producing L-malic acid mutant by sugar fermentation[J].Industrial Microbiology,2003,33(1):27-30. |
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| Authors: | LIU Jian-jun ZHAO Xiang-ying TIAN Yan-jun LI Pi-wu JIANG Lu-yan ZHANG Jia-xiang |
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| Affiliation: | LIU Jian-jun 1,2,ZHAO Xiang-ying 2,TIAN Yan-jun 2,LI Pi-wu 2,JIANG Lu-yan 2,ZHANG Jia-xiang 2 |
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| Abstract: | One strain Aspergillus flavus which could accumulate L-malic acid was isolated from soil samples. It was treated with U.V.,NTG, 60Co and DES, and an over-producing L-malic acid mutant Aspergillus flavus HA5800 by sugar fermentation was obtained.The L-malic acid yield and the conversion ratio of sugar to acid in shake-flask operation reached 72.0g/L and 74.22% respectively. There was no aflatoxin B 1 in the culture broth. |
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| Keywords: | L-malic acid Aspergillus flavus screening |
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