激光扫描共焦荧光显微镜的电子控制系统研究

介绍了激光扫描共焦荧光显微镜的电子控制系统,为了提高激光扫描器的定位精度,在系统中提出采用基于闭环象素时钟发生器的检流计振镜所固有的扫描非线性失真补偿方案,减少输出图象的枕形畸变,实现行扫描空间位置的均匀性。在以细分梯形波电流驱动反应式步进电机情况下,根据实测θ-i曲线,用反插法求得与步进电机运行非均匀性相补偿的相电流波,从而获得在小电流情况下高角度均匀的步进电机细分运行特性。

Study on electronic control system for laser scanning confocal fluorescence microscope

The electronic control system for laser scanning confocal fluorescence microscope is described in this paper.For improvement of the positing precision of laser scanner,a high accuracy scanning image sampling method,based on pixels clock generator,is adapted to correct the non-linear output image pincushion distortion caused by the rotational inertial of the e mechanical parts of laser scanner.In order to detect the scanning spatial position under the case of step motor driving,according to the experimentally measured I-θ curve,we can get the phase current wave form which compensates the non-linearity of I-θ curve by means of interpolation method.Therefore,the step motor can operate with high angle uniformity under the condition of low current mode.