淡豆豉与黑豆提取物抗癌细胞增殖作用及4种异黄酮成分的含量测定

目的:探讨淡豆豉和黑豆提取物对乳腺癌MCF-7细胞增殖的调节作用,并检测提取物中4种异黄酮成分的含量。方法:MTT法观察淡豆豉和黑豆提取物对乳腺癌细胞株MCF-7细胞增殖率的影响,Annexin/PI法流式细胞仪分析检测细胞的早期凋亡,高效液相色谱法检测淡豆豉和黑豆提取物中4种异黄酮成分大豆苷、染料木苷、大豆苷元和染料木素的含量变化。结果:淡豆豉和黑豆提取物对MCF-7细胞的增殖均有抑制作用且诱发癌细胞凋亡,但高浓度淡豆豉提取物的抑制癌细胞增殖作用明显高于黑豆(P0.05)。淡豆豉提取物中大豆苷、大豆苷元和染料木素的含量显著高于黑豆提取物(P0.01),但染料木苷的含量未见明显变化。结论:淡豆豉提取物与黑豆相比具有较强的抑制癌细胞增殖作用,淡豆豉中含量较高的异黄酮成分大豆苷、大豆苷元和染料木素,可能是其较强的抗癌细胞增殖作用的物质基础。

Anti-proliferative effects of Semen Sojae Praeparatum and Black Soybean extract and the content determination of four isoflavone components

Objective: To investigate the effect of Semen Sojae Praeparatum(SSP) and black soybean(BS) extracts on the MCF-7 cell proliferation,and determine of four isoflavone components content.Methods: MTT method was used to observe the effect of SSP and BS extract on the proliferation of breast cancer cell line MCF-7.Early cells apoptosis were detected by Annexin/PI flow cytometry analysis.Four isoflavones content(daidzin,genistin,daidzein and genistein) from SSP and BS extract were analyzed with HPLC assay.Results: Extracts of SSP and BS inhibited cell proliferation and induced apoptosis in MCF-7cancer cells.However,the high concentration of SSP extract resulted in a significantly inhibition than BS in cancer cell proliferation(P<0.05).Daidzin,daidzein and genistein in SSP extract were significantly higher than that of BS extract(P<0.01),but there is no change in genistin.Conclusion: SSP extract has strong inhibition of cancer cell proliferation compared with BS extract.Higher levels of daidzein,daidzin and genistein in SSP extract may be the mechanism for stronger effect of anti-cancer cell proliferation.