| 脐血造血干细胞分离方法的比较 |
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| 引用本文： | 鲍建芳,林敏,杨仁儿,夏大静,沈建根,邵传森.脐血造血干细胞分离方法的比较[J].浙江大学学报(医学版),1999,28(3):114-116. |
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| 作者姓名： | 鲍建芳 林敏 杨仁儿 夏大静 沈建根 邵传森 |
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| 作者单位： | 浙江医科大学免疫学教研室，宁波市海曙区天封医院 |
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| 摘 要： | 目的：探讨分离脐血造血干细胞的最佳方法。方法：采用Ｆｉｃｏｌ分层法、３％明胶自然沉降法及６％羟乙基淀粉自然沉降法。结果：３％明胶自然沉降法所获有核细胞（ＮＣ）密度高达９４．６０（±２３．８０）×１０５／ｍｌ，ＣＦＵＧＭ数为６４．３０（±１２．１８）／１０５ＮＣ，ＣＤ３４＋细胞数为１．２１（±０．３７）×１０５／ｍｌ，均较其它两种方法为高（Ｐ＜０．０５）。结论：３％明胶自然沉降法是一种较理想的分离脐血造血干细胞的方法。
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| 关 键 词： | 脐带血 CD34 造血细胞 细胞分离 |
Comparison of the Methods for Separating Hematopoietic Stem Cells from Umbilical Cord
Blood |
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| Bao Jianfang,Lin Min,Yang Rener,et al.Comparison of the Methods for Separating Hematopoietic Stem Cells from Umbilical Cord
Blood[J].Journal of Zhejiang University(Medical Sciences),1999,28(3):114-116. |
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| Authors: | Bao Jianfang Lin Min Yang Rener |
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| Abstract: | Objective: To find out an optimal method of separating
hematopoietic stem cells (HSC) from umbilical cord blood. Methods: Density gradient
centrifugation(FicollHypaque),3% gelatin natural sedimentation and 6% hydroxyethyl starch
natural sedimentation were compared. Results: The number of nucleate cells,CFUGM and
CD34 cells were 94.60(23.80)105/ml,64.30(12.18)/105 NC and 1.21(0.37)105/ml respectively by
3% gelatin natural sedimentation.These results were better than that from the other two methods
(P<0.05). Conclusion: Three per cent gelatin natural sedimentation is an optimal umethod for
separating HSC from umbilical cord blood. |
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| Keywords: | Umbilical cord blood CD34 Hematopoietic cell Cell separation |
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