同种异体胚胎干细胞移植治疗小鼠缺氧缺血性脑损伤

目的研究ESc来源的神经前体细胞移植入HIBD脑内后的迁移、分化,以探讨细胞移植治疗的机制。方法取7 d龄C57小鼠,制作HIBD动物模型。术后第2～3天,将ESc来源神经前体细胞植入小鼠损伤侧脑室,同时设对照组(只注射PBS)和正常组。然后对小鼠脑组织进行病理学、PCR、X-gal染色及免疫荧光组织化学检测。结果ESc来源神经前体细胞移植入HIBD脑内后,长期存在于脑内,并迁移、分布于受损的海马,构成海马结构,经进一步免疫荧光检测可分化为NF阳性的神经元。结论ESc来源神经前体细胞移植入HIBD脑内后,能迁移至损伤的海马,分化为神经元,替代损伤或坏死的神经细胞,这可能是其发挥治疗作用的机制之一。

Embryonic stem cells in the treatment of hypoxic-ischemic brain damage in mice

Objective To investigate the differentiation of neural progenitor cells originated from embryonic stem cells in the hippocampus after transplanted into the hypoxic ischemically damaged brain in mice.Method Seven-day-old hypoxie-isehemic brain damage model was produced.The embryonic stem(ES)cell-derived cells or PBS were transplanted into the lateral ventricle of the hypoxic ischemically damaged brain in mouse.After transplantation,PCR analysis,HE staining,X-gal staining and immunofluorescence were used to investigate the survivance and differentiation of the cells in the hippocampus.Results After transplantation,the ES cell-derived cells survived and differentiated into the NF positive cells instead of the glial fibrilliary protein(GFAP)positive cells in the hippocampus of the hypoxic ischemically damaged brain.Conclusions After transplantation,neural progenitor cells originated from embryonic stem cells could survive in the hippocampus and differentiate into the neurons.This founding may be the one of mechanism of the therapeutic effect of embryonic stem cells on the hypoxic ischemically damaged brain in newborns.