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Effect of gums on viability and β‐galactosidase activity of Lactobacillus spp. in milk drink during refrigerated storage
Authors:Bernice D Karlton‐Senaye  Reza Tahergorabi  Valerie L Giddings  Salam A Ibrahim
Affiliation:1. Energy and Environmental Systems, North Carolina Agricultural and Technical State University, Greensboro, NC, USA;2. Department of Family and Consumer Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USA
Abstract:The viability and β‐galactosidase activity of four Lactobacillus strains in milk drink containing gums during 28 days of refrigerated storage at 4 °C were assessed. The population of Lactobacillus rhamnosus GGB101 and Lactobacillus rhamnosus GGB103 were maintained, whereas the population of Lactobacillus reuteri DSM20016 and Lactobacillus reuteri SD2112 significantly decreased. The recommended level of 6 log CFU g?1 was exceeded for all tested trains throughout storage. The highest viable number of Lactobacillus rhamnosus GGB103 (8.76 ± 0.03 log CFU mL?1) was obtained in the product containing carrageenan–maltodextrin. The addition of guar–locust bean–carrageenan led to 20‐fold increase in the level of β‐galactosidase activity for L. rhamnosus GGB101 (1208 ± 2.12 Miller units mL?1) compared to the control (61 ± 2.83 Miller units mL?1). Our results suggested that gums could be added to milk to improve viability and enhance β‐galactosidase activity of Lactobacillus.
Keywords:Gums  Lactobacillus  lactose intolerance  polysaccharides  prebiotics  probiotics  viability  β  ‐galactosidase
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