| 柱前衍生高效液相色谱法测定门冬氨酸的含量 |
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| 引用本文： | 杨颖,阎秀美,陈乃江.柱前衍生高效液相色谱法测定门冬氨酸的含量[J].中国药业,2013(24):30-31. |
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| 作者姓名： | 杨颖 阎秀美 陈乃江 |
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| 作者单位： | [1]江苏省连云港市药品检验所,江苏连云港222000 [2]江苏德源药业有限公司,江苏连云港222000 |
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| 摘 要： | 目的建立测定门冬氨酸含量的异硫氰酸苯酯柱前衍生化方法。方法采用高效液相色谱法测定门冬氨酸的含量。色谱柱为Phe—nomenexGeminiC18柱（250mmX4．6mm，5μm），流动相A为12．5mmol／L磷酸钠溶液（用磷酸调pH至6．8）、B为乙腈-水（80：20），梯度洗脱，流速为1．0mL／min，检测波长为254nm。结果门冬氨酸质量浓度在50．0～800．0μg／mL范围内与峰面积呈良好的线性关系（r=0．9998），平均回收率为99．18％，RSD=1．74％（n=9）。结论该方法准确、专属性强，稳定且无干扰，可用于门冬氨酸的含量测定。
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| 关 键 词： | 门冬氨酸 柱前衍生 反相高效液相色谱法 |
Determination of Aspartic Acid with precolumn derivatization |
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| Affiliation: | Yang Ying1,Yan Xiumei2, Chert Naijiang1 (1. Lianyungang Municipal Institute for Drug Control, Lianyungang, Jiangsu, China 222000; 2. Jiangsu Deyuan Pharmaceutical Co. , Ltd. , Lianyungang, Jiangsu, China 222000) |
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| Abstract: | Objective To establish a high performance liquid chroatography（HPLC） method with precolumn derivatization for the deter- mination of aspartic acid content. Method The HPLC method was conducted on the Phenomenex Gemini C~8 column（250 mm x4. 6 mm, 5 ~m） with the mobile phase A of 12. 5 mmol/L sodium phosphate solution （adjusting pH to 6.8 with phosphoric acid） and the mobile phase B of aeetonitrile-water （80：20）,the gradient elution was used. The flow rate was 1.0 mL/min;the detection wavelength was 254 nm. Results The mass concentration of aspartic acid in the range of 50. 0- 800.0 ~xg/mL had good linear relationship with the peak area （r = 0. 999 8）,the average recovery rate was 99. 87%, RSD = 1. 741% （n = 9）. Conclusion This method is accurate with strong specificity,stability and no interference, which be used for the determination of aspartic acid. |
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| Keywords: | aspartic acid precolumn derivatization RP - HPLC |
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