利用组织芯片分析肝细胞肝癌中Caspase-9与Ki-67的表达及意义

目的 研究Cagpage-9和Ki-67在肝细胞肝癌(HCC)的表达及与临床病理参数之间的关系.方法 利用组织芯片技术和免疫组织化学方法检测76例HCC组织和38例正常肝组织中Cagpage-9和Ki-67的表达情况.结果 Cagpage-9在HCC组织中的表达阳性率(55.3%)低于正常肝组织(78.9%)(P＜0.05),Cagpage-9在HCC组织的表达与组织分化有关(P＜0.05);Ki-67在HCC组织中的表达阳性率(53.9%)高于正常肝组织(13.2%)(P＜0.01),Ki-67在HCC组织的表达与组织分化和转移有关(P＜0.05);在HCC组织中Cagpage-9与Ki-67的表达呈负相关(r=-0.300,P=0.008).结论 HCC中Cagpage-9的低表达与Ki-67的高表达可能共同参与HCC中细胞凋亡与增殖的病理过程,在肿瘤的发生、发展过程中起重要作用.

Expression and clinical significance of Caspase-9 and Ki-67 In hepatocellular carcinoma

Objective To observe the expression and clinical significance of Cagpage-9 and Ki-67 in hepato-cellular carcinoma(HCC).Methods Expression of Cagpage-9 and Ki-67 was detected by tissue microarray and immunohistochemical method in 76 HCC tissues and 38 normal liver tissues.Results The expressions of Caspage-9 in HCC tissues(55.3%)was significantly lower than that in normal tissues(78.9%)(P＜0.05)and was correlated with Edmondson,s histological grade of carcinoma(P＜0.05),but there was no correlation among patients,age,gender,diameter,metastasis and HBsAg expression(P＞0.05).The expression rate of Ki-67 in HCC tissues(53.9%)was significantly higher than in normal liver tissues(13.2%)(P＜0.01),and was correlated with Edmondson,s histological grade and metastasis of carcinoma(P＜0.05).There was no correlation among patients,age,sex and HBsAg expression(P＞0.05).Meanwhile,expressions of Caspage-9 wag negative correlated with Ki-67(r=-0.300,P=0.008).Conclusion Low Caspage-9 expression and high Ki-67 expression may involved in the pathological process of cell apoptosis and proliferation,and play an important role in occurrence and progression of hepatocellular carcinoma.