| 蜂毒素对肝癌细胞磷脂酶A_2的影响 |
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| 引用本文： | 张晨,付大鹏,韩大跃,李勇,凌昌全.蜂毒素对肝癌细胞磷脂酶A_2的影响[J].解放军医学高等专科学校学报,2010(2):259-260. |
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| 作者姓名： | 张晨 付大鹏 韩大跃 李勇 凌昌全 |
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| 作者单位： | [1]解放军第210医院肿瘤科,辽宁大连116021 [2]解放军第二军医大学长海医院中医科,上海200433 |
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| 摘 要： | 目的观察蜂毒素诱导细胞凋亡及对胞质型细胞磷脂酶A2（cPLA2）的影响。方法肝癌细胞BEL-7402体外培养,采用MTT法了解蜂毒素对肝癌细胞增殖的影响,用流式细胞术测定细胞凋亡,采用RT-PCR检测cPLA2的表达。结果蜂毒素在体外能够抑制肝癌细胞的增殖活性,并有诱导细胞凋亡的作用,RT-PCR检测显示,蜂毒素mRNA水平均具有激活cPLA2的作用。结论蜂毒素具有诱导肝癌凋亡及激活cPLA2的生物活性。
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| 关 键 词： | 蜂毒素 肝肿瘤 凋亡 磷脂酶A2 |
Effect of melittin on cytosolic phospholipase A_2 in hepatocarcinoma |
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| Zhang Chen,Fu Da-peng,Han Da-yue,Li Yong,Ling Chang-quan.Effect of melittin on cytosolic phospholipase A_2 in hepatocarcinoma[J].Clinical Journal of Medical Officer,2010(2):259-260. |
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| Authors: | Zhang Chen Fu Da-peng Han Da-yue Li Yong Ling Chang-quan |
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| Affiliation: | 1.Department of Oncology,PLA No.210 Hospital,Dalian Liaoning 116021,China;2.Department of Traditional Chinese Medicine,Changhai Hospital,Second Military Medical University,Shanghai 200433,China) |
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| Abstract: | Objective To study the effect of melittin on apoptosis and cytosolic phospholipase A2（cPLA2） of human hepatoma cell line in vitro.Methods BEL-7402 cell line was cultured invitro,and treated with melittin.The inhibition of proliferation,apoptosis and cPLA2 were detected by MTT assay,Flow cytometry,and RT-PCR,respectively.Results Melittin inhibited cell proliferation and induced apoptosis in vitro.Anther experimental outcome showed that cPLA2 was activated in mRNA level.Conclusion It is suggested that melittin can activate cPLA2 and induce apoptosis in hepatocarcinoma. |
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| Keywords: | melittin hepatocarcinoma apoptosis cytosolic phospholipase A2 |
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