| 六味地黄生物制剂中多糖脱蛋白方法对比 |
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| 引用本文： | 吴思,丘婷,陈朋,李天河,汤佩莲,赵越.六味地黄生物制剂中多糖脱蛋白方法对比[J].中国实验方剂学杂志,2012,18(4):56-59. |
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| 作者姓名： | 吴思 丘婷 陈朋 李天河 汤佩莲 赵越 |
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| 作者单位： | 广东药学院中药学院,广州 ,510006 |
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| 基金项目： | 广东省自然科学基金重点项目(No.825022401000006);广州市科技局项目(No.2008Z1-E381) |
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| 摘 要： | 目的:对六味地黄生物制剂粗多糖中蛋白质的去除方法进行比较研究,探讨不同方法对多糖的提取率及纯度的影响.方法:利用Sevage法、TCA-正丁醇沉淀法、鞣酸沉淀法对六味地黄生物制剂脱蛋白,通过蛋白清除率和多糖保存率比较3种方法的优劣.结果:TCA-正丁醇法脱蛋白时效果最好,最佳条件为TCA-正丁醇试剂的体积比1∶2,TCA-正丁醇试剂的配比1∶5,振摇时间30 min,静置时间1.0h,多糖脱蛋白效率最高,多糖保存率最大.结论:TCA-正丁醇法是目前六味地黄生物制剂脱蛋白效果最好的工艺.
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| 关 键 词： | 多糖 脱蛋白 Sevage法 TCA-正丁醇法 鞣酸法 |
Methods Comparative of Removal of Protein for Polysaccharide from Liuwei Dihuang Biological Preparation |
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| WU Si,QIU Ting,CHEN Peng,LI Tian-he,TANG Pei-lian and ZHAO Yue.Methods Comparative of Removal of Protein for Polysaccharide from Liuwei Dihuang Biological Preparation[J].China Journal of Experimental Traditional Medical Formulae,2012,18(4):56-59. |
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| Authors: | WU Si QIU Ting CHEN Peng LI Tian-he TANG Pei-lian and ZHAO Yue |
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| Affiliation: | College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China |
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| Abstract: | Objective:To comparative study on removal method of protein for polysaccharide from Liuwei Dihuang biological preparation,and investigate effect on yield and purity of polysaccharide by different methods.Method:Sevage method,TCA-butanol precipitation method and tannin acid precipitation method were taken to deproteinized of Liuwei Dihuang biological preparation,and compared effect of three deproteinization methods by deproteinization rate and polysaccharides retention rate.Result:Deproteinizationhe effect of TCA-butanol precipitation method was the best,optimum conditions were:volume ratio of TCA-butanol reagent was 1∶2,ratio of TCA-butanol reagents was 1∶5,vibration time was 30 min,standing for 1.0 h,which showed highest efficient of deproteinization for polysaccharides and higher polysaccharides retention rate.Conclusion:TCA-butanol method was optimum technology of removing protein from Liuwei Dihuang biological preparation. |
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| Keywords: | polysaccharide deproteinization Sevage method TCA-butanol method tannin acid method |
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