湿度盐度pH协同驱动锡林河景观疣微菌群空间异质性

[目的]为了阐明锡林河不同景观要素间疣微菌群的空间分布异质性及环境驱动力.[方法]本文基于生境及微地形差异选择水生的河床中心、河床边缘与牛轭湖床,湿生的低河漫滩与高河漫滩,旱生的低阶地与高阶地等景观要素,基于16S rRNA基因高通量测序技术研究不同景观要素土壤疣微菌群的景观异质性,结合土壤湿度等环境变量研究其驱动力....

Moisture, salinity and pH co-driving spatial heterogeneity of Verrucomicrobial populations in Xilin River landscape

[Objective] In this paper, our main goal was to clarify the spatial heterogeneity and environmental driver of Verrucomicrobial populations from different landscape components in Xilin River basin. [Methods] Based on the difference of habitat and microtopography, we selected the landscape components of aquatic riverbed center, riverbed edge and oxbow lakebed, hygric low floodplain and high floodplain, xeric low terrace and high terrace. Then we employed 16S rRNA gene high-throughput sequencing to study the landscape heterogeneity of soil Verrucomicrobial populations from different landscape components. We also explored environmental variables driving landscape heterogeneity of soil Verrucomicrobial populations combining with environmental variables (e.g. soil moisture). [Results] Spatial distribution of Verrucomicrobial populations showed convergent characteristics within landscape components and divergent characteristics between landscape components. The members of Chthoniobacter from Spartobacteria class and Prosthecobacter from Verrucomicrobiae class were mainly distributed in the aquatic riverbed center (RC), showing significant positive correlation with sand particle (P<0.05). Those of Opitutae class were mainly distributed in hygric floodplain, showing positive correlation with pH (P<0.05 or P>0.05). DA101_soil_group members of Spartobacteria class were mainly distributed in the xeric terrace, showing significant positive correlation with salinity and nutrients (P<0.01). Variation partitioning analyses of single, two groups and three groups of environmental variables showed that moisture, salinity and pH explained 24.7% & 21.4%, 24.3% & 22.7% and 23.1% & 20.8% of landscape heterogeneity of Verrucomicrobial populations, respectively. [Conclusion] Verrucomicrobial populations demonstrated landscape heterogeneity in Xilin River basin. Members of Chthoniobacter and Prosthecobacter were indicators of the riverbed center landscape component, DA101_soil_group was the indicator of the terrace landscape components. The combination of moisture, salinity with pH co-drove the landscape heterogeneity of Verrucomicrobial populations.